Temporal and spatial expression analyses of TrEhod40, TrEnod5 and a novel early nodulin in white clover roots and nodules

MA Crockard, AJ Bjourson, MG Pulvirenti, JE Cooper

Research output: Contribution to journalArticle

Abstract

Using differential display RT-PCR on mRNA populations from white clover roots challenged with a mock-inoculation or the microsymbiont, Rhizobium leguminosarum biovar trifolii ANU843, a number of expressed sequence tags (ESTs), specific to the early stages of symbiosis, were identified. Similarity searches of many of these ESTs identified them as novel, with no significant sequence homologues. Of the white clover cDNA fragments with homologies to sequences in EMBL databases, several early nodulins were identified and further characterized by RT-PCR and in situ hybridization analyses to provide detailed temporal and spatial expression profiles. Here, we report on the analyses of a clover Enod40 homologue and a new early nodulin precursor (DD17), with 3' homology to Enod8, previously only identified in Medicago species. An arabinogalactan-like protein, highly homologous to previously reported Enod5s from other legumes. was also characterized and used as a standard for all expression analyses, following its isolation through degenerate-primer RT-PCR. Published by Elsevier Science Ireland Ltd.
LanguageEnglish
Pages1161-1170
JournalPlant Science
Volume161
Issue number6
Publication statusPublished - Nov 2001

Fingerprint

nodulins
Trifolium repens
reverse transcriptase polymerase chain reaction
expressed sequence tags
Rhizobium leguminosarum bv. trifolii
arabinogalactans
microsymbionts
Medicago
Ireland
in situ hybridization
symbiosis
legumes
proteins

Cite this

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title = "Temporal and spatial expression analyses of TrEhod40, TrEnod5 and a novel early nodulin in white clover roots and nodules",
abstract = "Using differential display RT-PCR on mRNA populations from white clover roots challenged with a mock-inoculation or the microsymbiont, Rhizobium leguminosarum biovar trifolii ANU843, a number of expressed sequence tags (ESTs), specific to the early stages of symbiosis, were identified. Similarity searches of many of these ESTs identified them as novel, with no significant sequence homologues. Of the white clover cDNA fragments with homologies to sequences in EMBL databases, several early nodulins were identified and further characterized by RT-PCR and in situ hybridization analyses to provide detailed temporal and spatial expression profiles. Here, we report on the analyses of a clover Enod40 homologue and a new early nodulin precursor (DD17), with 3' homology to Enod8, previously only identified in Medicago species. An arabinogalactan-like protein, highly homologous to previously reported Enod5s from other legumes. was also characterized and used as a standard for all expression analyses, following its isolation through degenerate-primer RT-PCR. Published by Elsevier Science Ireland Ltd.",
author = "MA Crockard and AJ Bjourson and MG Pulvirenti and JE Cooper",
year = "2001",
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language = "English",
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pages = "1161--1170",
journal = "Plant Science",
issn = "0168-9452",
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}

Temporal and spatial expression analyses of TrEhod40, TrEnod5 and a novel early nodulin in white clover roots and nodules. / Crockard, MA; Bjourson, AJ; Pulvirenti, MG; Cooper, JE.

In: Plant Science, Vol. 161, No. 6, 11.2001, p. 1161-1170.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Temporal and spatial expression analyses of TrEhod40, TrEnod5 and a novel early nodulin in white clover roots and nodules

AU - Crockard, MA

AU - Bjourson, AJ

AU - Pulvirenti, MG

AU - Cooper, JE

PY - 2001/11

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AB - Using differential display RT-PCR on mRNA populations from white clover roots challenged with a mock-inoculation or the microsymbiont, Rhizobium leguminosarum biovar trifolii ANU843, a number of expressed sequence tags (ESTs), specific to the early stages of symbiosis, were identified. Similarity searches of many of these ESTs identified them as novel, with no significant sequence homologues. Of the white clover cDNA fragments with homologies to sequences in EMBL databases, several early nodulins were identified and further characterized by RT-PCR and in situ hybridization analyses to provide detailed temporal and spatial expression profiles. Here, we report on the analyses of a clover Enod40 homologue and a new early nodulin precursor (DD17), with 3' homology to Enod8, previously only identified in Medicago species. An arabinogalactan-like protein, highly homologous to previously reported Enod5s from other legumes. was also characterized and used as a standard for all expression analyses, following its isolation through degenerate-primer RT-PCR. Published by Elsevier Science Ireland Ltd.

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