Targeted Chemo-Sonodynamic Therapy Treatment of Breast Tumours Using Ultrasound Responsive Microbubbles Loaded with Paclitaxel, Doxorubicin and Rose Bengal.

J Callan, Keiran Logan, Federica Foglietta, Heather Nesbitt, Yingjie Sheng, Thomas McKaig, Sukanta Kamila, Jinhui Gao, Nikolitsa Nomikou, Bridgeen Callan, Anthony P. McHale

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mastectomy is a common surgical treatment used in the management of breast cancer but has associated physical and psychological consequences for the patient. Breast conservation surgery (BCS) is an alternative to mastectomy but is only possible when the tumour is of an appropriate size. Neo-adjuvant chemotherapy has been successfully used to downstage tumours and increase the number of patients eligible for BCS. However, the chemotherapies used in this approach are non-targeted and often result in significant side effects to the patient. In this manuscript, we evaluate the potential of ultrasound targeted microbubble destruction (UTMD) to deliver Rose Bengal-mediated sonodynamic therapy (SDT) in combination with paclitaxel (PTX) and doxorubicin (Dox) chemotherapy as a potential treatment for breast cancer. Efficacy of the combined treatment was determined in a three-dimensional (3D) spheroid model of human breast cancer and in a murine model of the disease bearing subcutaneous MCF-7 tumours. The results demonstrated a significant reduction in both the cell viability of spheroids and tumour volume following treatment with the drug loaded microbubbles and ultrasound compared to targets treated with the drug loaded microbubbles alone or a Cremophor EL suspension of PTX and Dox. In addition, the weight of animals that received the microbubble treatment was unchanged throughout the study while a reduction of 12.1% was observed for animals treated with a Cremophor suspension of PTX/Dox. These results suggest that UTMD-mediated chemo-sonodynamic therapy is an efficacious and well tolerated approach for the treatment of breast cancer.
LanguageEnglish
Pages224-231
Number of pages8
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume139
Early online date5 Apr 2019
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Rose Bengal
Microbubbles
Paclitaxel
Doxorubicin
Breast Neoplasms
Mastectomy
Therapeutics
Suspensions
Breast
Drug Therapy
Neoplasms
Adjuvant Chemotherapy
Tumor Burden
Pharmaceutical Preparations
Cell Survival
Psychology
Weights and Measures

Keywords

  • Chemotherapy
  • sonodynamic therapy
  • microbubbles
  • ultrasound targeted microbubble destruction
  • breast cancer
  • MCF7
  • Ultrasound targeted microbubble destruction
  • MCF-7
  • Sonodynamic therapy
  • Microbubbles
  • Breast cancer

Cite this

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title = "Targeted Chemo-Sonodynamic Therapy Treatment of Breast Tumours Using Ultrasound Responsive Microbubbles Loaded with Paclitaxel, Doxorubicin and Rose Bengal.",
abstract = "Mastectomy is a common surgical treatment used in the management of breast cancer but has associated physical and psychological consequences for the patient. Breast conservation surgery (BCS) is an alternative to mastectomy but is only possible when the tumour is of an appropriate size. Neo-adjuvant chemotherapy has been successfully used to downstage tumours and increase the number of patients eligible for BCS. However, the chemotherapies used in this approach are non-targeted and often result in significant side effects to the patient. In this manuscript, we evaluate the potential of ultrasound targeted microbubble destruction (UTMD) to deliver Rose Bengal-mediated sonodynamic therapy (SDT) in combination with paclitaxel (PTX) and doxorubicin (Dox) chemotherapy as a potential treatment for breast cancer. Efficacy of the combined treatment was determined in a three-dimensional (3D) spheroid model of human breast cancer and in a murine model of the disease bearing subcutaneous MCF-7 tumours. The results demonstrated a significant reduction in both the cell viability of spheroids and tumour volume following treatment with the drug loaded microbubbles and ultrasound compared to targets treated with the drug loaded microbubbles alone or a Cremophor EL suspension of PTX and Dox. In addition, the weight of animals that received the microbubble treatment was unchanged throughout the study while a reduction of 12.1{\%} was observed for animals treated with a Cremophor suspension of PTX/Dox. These results suggest that UTMD-mediated chemo-sonodynamic therapy is an efficacious and well tolerated approach for the treatment of breast cancer.",
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Targeted Chemo-Sonodynamic Therapy Treatment of Breast Tumours Using Ultrasound Responsive Microbubbles Loaded with Paclitaxel, Doxorubicin and Rose Bengal. / Callan, J; Logan, Keiran; Foglietta, Federica; Nesbitt, Heather; Sheng, Yingjie; McKaig, Thomas; Kamila, Sukanta; Gao, Jinhui; Nomikou, Nikolitsa; Callan, Bridgeen; McHale, Anthony P.

Vol. 139, 01.06.2019, p. 224-231.

Research output: Contribution to journalArticle

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T1 - Targeted Chemo-Sonodynamic Therapy Treatment of Breast Tumours Using Ultrasound Responsive Microbubbles Loaded with Paclitaxel, Doxorubicin and Rose Bengal.

AU - Callan, J

AU - Logan, Keiran

AU - Foglietta, Federica

AU - Nesbitt, Heather

AU - Sheng, Yingjie

AU - McKaig, Thomas

AU - Kamila, Sukanta

AU - Gao, Jinhui

AU - Nomikou, Nikolitsa

AU - Callan, Bridgeen

AU - McHale, Anthony P.

PY - 2019/6/1

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KW - MCF-7

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