Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease

S Finnegan, J Robson, C Scaife, C McAllister, SR Pennington, David Gibson, ME Rooney

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

INTRODUCTION: Juvenile idiopathic arthritis (JIA) is the most common rheumatological disease of childhood with a prevalence of around 1 in 1000. Without appropriate treatment it can have devastating consequences including permanent disability from joint destruction and growth deformities. Disease aetiology remains unknown. Investigation of disease pathology at the level of the synovial membrane is required if we want to begin to understand the disease at the molecular and biochemical level. The synovial membrane proteome from early disease-stage, treatment naive JIA patients was compared between polyarticular and oligoarticular subgroups.METHODS: Protein was extracted from 15 newly diagnosed, treatment naive JIA synovial membrane biopsies and separated by two dimensional fluorescent difference in-gel electrophoresis. Proteins displaying a two-fold or greater change in expression levels between the two subgroups were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry with expression further verified by Western blotting and immunohistochemistry.RESULTS: Analysis of variance analysis (P
LanguageEnglish
Article numberR8
JournalArthritis Research and Therapy
Volume16
Issue number1
DOIs
Publication statusPublished - 13 Jan 2014

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Juvenile Arthritis
Synovial Membrane
Membrane Proteins
Analysis of Variance
Far-Western Blotting
Proteome
Electrophoresis
Mass Spectrometry
Proteins
Lasers
Therapeutics
Joints
Gels
Immunohistochemistry
Pathology
Biopsy
Growth

Cite this

Finnegan, S ; Robson, J ; Scaife, C ; McAllister, C ; Pennington, SR ; Gibson, David ; Rooney, ME. / Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease. In: Arthritis Research and Therapy. 2014 ; Vol. 16, No. 1.
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Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease. / Finnegan, S; Robson, J; Scaife, C; McAllister, C; Pennington, SR; Gibson, David; Rooney, ME.

In: Arthritis Research and Therapy, Vol. 16, No. 1, R8, 13.01.2014.

Research output: Contribution to journalArticle

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AU - Finnegan, S

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AU - Rooney, ME

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AB - INTRODUCTION: Juvenile idiopathic arthritis (JIA) is the most common rheumatological disease of childhood with a prevalence of around 1 in 1000. Without appropriate treatment it can have devastating consequences including permanent disability from joint destruction and growth deformities. Disease aetiology remains unknown. Investigation of disease pathology at the level of the synovial membrane is required if we want to begin to understand the disease at the molecular and biochemical level. The synovial membrane proteome from early disease-stage, treatment naive JIA patients was compared between polyarticular and oligoarticular subgroups.METHODS: Protein was extracted from 15 newly diagnosed, treatment naive JIA synovial membrane biopsies and separated by two dimensional fluorescent difference in-gel electrophoresis. Proteins displaying a two-fold or greater change in expression levels between the two subgroups were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry with expression further verified by Western blotting and immunohistochemistry.RESULTS: Analysis of variance analysis (P

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