Survival of Campylobacter jejuni in waterborne protozoa

WJ Snelling, JP McKenna, DM Lecky, James Dooley

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The failure to reduce the Campylobacter contamination of intensively reared poultry may be partially due to Campylobacter resisting disinfection in water after their internalization by waterborne protozoa. Campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. An in vitro assay showed that C jejuni remained viable when internalized by Tetrahymena pyriformis and Acanthamoeba castellanii for significantly longer (up to 36 h) than when they were in purely a planktonic state. The internalized Campylobacter were also significantly more resistant to disinfection than planktonic organisms. Collectively, our results strongly suggest that protozoa in broiler drinking water systems can delay the decline of Campylobacter viability and increase Campylobacter disinfection resistance,. thus increasing the potential of Campylobacter to colonize broilers.
LanguageEnglish
Pages5560-5571
JournalApplied and Environmental Microbiology
Volume71
Issue number9
DOIs
Publication statusPublished - Sep 2005

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Campylobacter jejuni
Campylobacter
Protozoa
disinfection
drinking water
poultry
broiler chickens
Acanthamoeba castellanii
Tetrahymena pyriformis
Ciliophora
viability
organisms
assays
water

Cite this

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title = "Survival of Campylobacter jejuni in waterborne protozoa",
abstract = "The failure to reduce the Campylobacter contamination of intensively reared poultry may be partially due to Campylobacter resisting disinfection in water after their internalization by waterborne protozoa. Campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. An in vitro assay showed that C jejuni remained viable when internalized by Tetrahymena pyriformis and Acanthamoeba castellanii for significantly longer (up to 36 h) than when they were in purely a planktonic state. The internalized Campylobacter were also significantly more resistant to disinfection than planktonic organisms. Collectively, our results strongly suggest that protozoa in broiler drinking water systems can delay the decline of Campylobacter viability and increase Campylobacter disinfection resistance,. thus increasing the potential of Campylobacter to colonize broilers.",
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Survival of Campylobacter jejuni in waterborne protozoa. / Snelling, WJ; McKenna, JP; Lecky, DM; Dooley, James.

In: Applied and Environmental Microbiology, Vol. 71, No. 9, 09.2005, p. 5560-5571.

Research output: Contribution to journalArticle

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AU - Snelling, WJ

AU - McKenna, JP

AU - Lecky, DM

AU - Dooley, James

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AB - The failure to reduce the Campylobacter contamination of intensively reared poultry may be partially due to Campylobacter resisting disinfection in water after their internalization by waterborne protozoa. Campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. An in vitro assay showed that C jejuni remained viable when internalized by Tetrahymena pyriformis and Acanthamoeba castellanii for significantly longer (up to 36 h) than when they were in purely a planktonic state. The internalized Campylobacter were also significantly more resistant to disinfection than planktonic organisms. Collectively, our results strongly suggest that protozoa in broiler drinking water systems can delay the decline of Campylobacter viability and increase Campylobacter disinfection resistance,. thus increasing the potential of Campylobacter to colonize broilers.

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