Survival and distribution of cell-free SEF 21 of Salmonella enterica serovar Enteritidis in the stomach and various compartments of the rat gastrointestinal tract in vivo

Patrick Naughton, G Grant, M Sojka, S Bardocz, CJ Thorns, A Pusztai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rats were dosed for 6 days with purified SEF 21 fimbriae of Salmonella enterica serovar Enteritidis 10360. The levels of fimbriae in gut contents associated with tissues and in the faeces were quantified by direct non-competitive ELISA. SEF 21 was distributed throughout the gut. The majority was found in the large intestine where it was primarily in the luminal contents. In contrast, a high proportion of SEF 21 detected in the ileum, the main site of salmonella colonisation and invasion, was tissue-bound. Thus, purified SEF 21 survived intestinal passage and associated with the stomach and gastrointestinal tract in a pattern similar to that found with whole Salmonella cells.
LanguageEnglish
Pages1049-1054
JournalJournal of Medical Microbiology
Volume50
Issue number12
Publication statusPublished - Dec 2001

Fingerprint

fimbriae
Salmonella Enteritidis
gastrointestinal system
Salmonella
stomach
digestive system
rats
large intestine
ileum
feces
enzyme-linked immunosorbent assay
cells
tissues

Keywords

  • SEF 21
  • Salmonella
  • Type 1 fimbriae

Cite this

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title = "Survival and distribution of cell-free SEF 21 of Salmonella enterica serovar Enteritidis in the stomach and various compartments of the rat gastrointestinal tract in vivo",
abstract = "Rats were dosed for 6 days with purified SEF 21 fimbriae of Salmonella enterica serovar Enteritidis 10360. The levels of fimbriae in gut contents associated with tissues and in the faeces were quantified by direct non-competitive ELISA. SEF 21 was distributed throughout the gut. The majority was found in the large intestine where it was primarily in the luminal contents. In contrast, a high proportion of SEF 21 detected in the ileum, the main site of salmonella colonisation and invasion, was tissue-bound. Thus, purified SEF 21 survived intestinal passage and associated with the stomach and gastrointestinal tract in a pattern similar to that found with whole Salmonella cells.",
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Survival and distribution of cell-free SEF 21 of Salmonella enterica serovar Enteritidis in the stomach and various compartments of the rat gastrointestinal tract in vivo. / Naughton, Patrick; Grant, G; Sojka, M; Bardocz, S; Thorns, CJ; Pusztai, A.

In: Journal of Medical Microbiology, Vol. 50, No. 12, 12.2001, p. 1049-1054.

Research output: Contribution to journalArticle

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AU - Grant, G

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AU - Bardocz, S

AU - Thorns, CJ

AU - Pusztai, A

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