TY - JOUR
T1 - Surface-Adsorbed Long G-Quadruplex Nanowires Formed by G:C Linkages
AU - Troha, Tinkara
AU - Drevenšek-Olenik, Irena
AU - Webba da Silva, Mateus
AU - Spindler, Lea
PY - 2016/7/19
Y1 - 2016/7/19
N2 - G-quadruplexes connected into long, continuous nanostructures termed G-wires show properties superior to dsDNA when applied in nanotechnology. Using AFM imaging, we systematically studied surface adsorption of a set of G-rich oligonucleotides with GC-termini for their ability to form long G-wires through G:C pairing. We investigated the effects of increasing sequence length, the type of nucleotide in the side loops, and removal of the CG-3′ terminus. We found that sequences with adenine in the side loops most readily form G-wires. The role of magnesium as an efficient surface-anchoring ion was also confirmed. Conversely, as resolved from dynamic light scattering measurements, magnesium had no ability to promote G-quadruplex formation in solution. These insights may help in selecting prosperous candidates for construction of G-quadruplex based nanowires and to explore them for their electronic properties
AB - G-quadruplexes connected into long, continuous nanostructures termed G-wires show properties superior to dsDNA when applied in nanotechnology. Using AFM imaging, we systematically studied surface adsorption of a set of G-rich oligonucleotides with GC-termini for their ability to form long G-wires through G:C pairing. We investigated the effects of increasing sequence length, the type of nucleotide in the side loops, and removal of the CG-3′ terminus. We found that sequences with adenine in the side loops most readily form G-wires. The role of magnesium as an efficient surface-anchoring ion was also confirmed. Conversely, as resolved from dynamic light scattering measurements, magnesium had no ability to promote G-quadruplex formation in solution. These insights may help in selecting prosperous candidates for construction of G-quadruplex based nanowires and to explore them for their electronic properties
KW - nanowire
KW - quadruplex
KW - G-wires
KW - DNA nanotechnology
UR - http://pubs.acs.org/doi/abs/10.1021/acs.langmuir.6b01222
UR - https://pure.ulster.ac.uk/en/publications/surface-adsorbed-long-g-quadruplex-nanowires-formed-by-gc-linkage-3
U2 - 10.1021/acs.langmuir.6b01222
DO - 10.1021/acs.langmuir.6b01222
M3 - Article
SN - 1520-5827
VL - 32
SP - 7056
EP - 7063
JO - Langmuir
JF - Langmuir
IS - 28
ER -
