Sulphated CCK-8-like peptides in the neural ganglion of the protochordate Ciona intestinalis

J. M. Conlon; T. W. Schwartz; J. F. Rehfeld

doi:10.1016/0167-0115(88)90080-8

Sulphated CCK-8-like peptides in the neural ganglion of the protochordate Ciona intestinalis

J. M. Conlon
, T. W. Schwartz
, J. F. Rehfeld

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Immunochemical studies were carried out on extracts of the neural ganglion from the ascidian Ciona intestinalis in order to the characterize the peptide(s), which react with antibodies against the C-terminal sequence common for the mammalian hormones, cholecystokinin (CCK) and gastrin. Radioimmunoassays specific for the sulphotyrosyl-containing N-terminus of CCK-8, for the common α-carboxyamidated C-terminus and for gastrin were used to monitor gel chromatography and reverse-phase HPLC of the extracts. Only neutral extracts contained immunoreactive material (634 (524-785) pmol eqv.CCK-8/g) (mean and range, n = 4)). HPLC revealed a small peak eluting almost like CCK-8 and a larger peak eluting earlier. By subsequent gel chromatography the larger peak eluted in the same position as sulphated CCK-8. The material was recognized almost equally by the N- and C-terminal CCK radioimmunoassays, whereas the specific C-terminal gastrin radioimmunoassay did not measure the peptides. Treatment with arylsulphatase removed the binding to the antiserum specific for the sulphotyrosyl-containing sequence of CCK. The results indicate that the ganglion of Ciona intestinalis contains a tyrosyl-sulphated peptide resembling mammalian CCK-8.

Original language	English
Pages (from-to)	241-250
Number of pages	10
Journal	Regulatory Peptides
Volume	20
Issue number	3
DOIs	https://doi.org/10.1016/0167-0115(88)90080-8
Publication status	Published (in print/issue) - Mar 1988

Funding

This study was supported by the Stiftung Volkswagenwerk and the Danish Medical Research Council. The authors would like to thank the staff of the Kristineberg Marine Biology Station, Drs. Sture Falkmer, Ib Svane and Dr. M. Thorndyke, Department of Zoology, Royal Holloway and Bedford New College, London, U.K. for their help in collecting the specimens. We also thank Dr. J.S. Morley, Walton Hospital, Liverpool, U.K. for generous support with peptide analogues necessary to characterize the specificity of our antisera. The skilful technical assistance of Susanne Hummelgaard is gratefully acknowledged.

Keywords

Cholecystokinin (CCK)
Gastrin
Neural ganglion
Protochordate
Sequence-specific radioimmunoassay

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title = "Sulphated CCK-8-like peptides in the neural ganglion of the protochordate Ciona intestinalis",

abstract = "Immunochemical studies were carried out on extracts of the neural ganglion from the ascidian Ciona intestinalis in order to the characterize the peptide(s), which react with antibodies against the C-terminal sequence common for the mammalian hormones, cholecystokinin (CCK) and gastrin. Radioimmunoassays specific for the sulphotyrosyl-containing N-terminus of CCK-8, for the common α-carboxyamidated C-terminus and for gastrin were used to monitor gel chromatography and reverse-phase HPLC of the extracts. Only neutral extracts contained immunoreactive material (634 (524-785) pmol eqv.CCK-8/g) (mean and range, n = 4)). HPLC revealed a small peak eluting almost like CCK-8 and a larger peak eluting earlier. By subsequent gel chromatography the larger peak eluted in the same position as sulphated CCK-8. The material was recognized almost equally by the N- and C-terminal CCK radioimmunoassays, whereas the specific C-terminal gastrin radioimmunoassay did not measure the peptides. Treatment with arylsulphatase removed the binding to the antiserum specific for the sulphotyrosyl-containing sequence of CCK. The results indicate that the ganglion of Ciona intestinalis contains a tyrosyl-sulphated peptide resembling mammalian CCK-8.",

keywords = "Cholecystokinin (CCK), Gastrin, Neural ganglion, Protochordate, Sequence-specific radioimmunoassay",

author = "Conlon, \{J. M.\} and Schwartz, \{T. W.\} and Rehfeld, \{J. F.\}",

year = "1988",

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AU - Conlon, J. M.

AU - Schwartz, T. W.

AU - Rehfeld, J. F.

PY - 1988/3

Y1 - 1988/3

N2 - Immunochemical studies were carried out on extracts of the neural ganglion from the ascidian Ciona intestinalis in order to the characterize the peptide(s), which react with antibodies against the C-terminal sequence common for the mammalian hormones, cholecystokinin (CCK) and gastrin. Radioimmunoassays specific for the sulphotyrosyl-containing N-terminus of CCK-8, for the common α-carboxyamidated C-terminus and for gastrin were used to monitor gel chromatography and reverse-phase HPLC of the extracts. Only neutral extracts contained immunoreactive material (634 (524-785) pmol eqv.CCK-8/g) (mean and range, n = 4)). HPLC revealed a small peak eluting almost like CCK-8 and a larger peak eluting earlier. By subsequent gel chromatography the larger peak eluted in the same position as sulphated CCK-8. The material was recognized almost equally by the N- and C-terminal CCK radioimmunoassays, whereas the specific C-terminal gastrin radioimmunoassay did not measure the peptides. Treatment with arylsulphatase removed the binding to the antiserum specific for the sulphotyrosyl-containing sequence of CCK. The results indicate that the ganglion of Ciona intestinalis contains a tyrosyl-sulphated peptide resembling mammalian CCK-8.

AB - Immunochemical studies were carried out on extracts of the neural ganglion from the ascidian Ciona intestinalis in order to the characterize the peptide(s), which react with antibodies against the C-terminal sequence common for the mammalian hormones, cholecystokinin (CCK) and gastrin. Radioimmunoassays specific for the sulphotyrosyl-containing N-terminus of CCK-8, for the common α-carboxyamidated C-terminus and for gastrin were used to monitor gel chromatography and reverse-phase HPLC of the extracts. Only neutral extracts contained immunoreactive material (634 (524-785) pmol eqv.CCK-8/g) (mean and range, n = 4)). HPLC revealed a small peak eluting almost like CCK-8 and a larger peak eluting earlier. By subsequent gel chromatography the larger peak eluted in the same position as sulphated CCK-8. The material was recognized almost equally by the N- and C-terminal CCK radioimmunoassays, whereas the specific C-terminal gastrin radioimmunoassay did not measure the peptides. Treatment with arylsulphatase removed the binding to the antiserum specific for the sulphotyrosyl-containing sequence of CCK. The results indicate that the ganglion of Ciona intestinalis contains a tyrosyl-sulphated peptide resembling mammalian CCK-8.

KW - Cholecystokinin (CCK)

KW - Gastrin

KW - Neural ganglion

KW - Protochordate

KW - Sequence-specific radioimmunoassay

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ER -

Sulphated CCK-8-like peptides in the neural ganglion of the protochordate Ciona intestinalis

Abstract

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