Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis

C Millar; J Moore; C Lowery; K McCorry; J Dooley

doi:10.1078/1438-4639-00090

Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis

C Millar
, J Moore
, C Lowery
, K McCorry
, J Dooley

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Abstract A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets.

Original language	English
Pages (from-to)	191-194
Journal	International Journal of Hygiene and Environmental Health
Volume	204
Issue number	2-3
DOIs	https://doi.org/10.1078/1438-4639-00090
Publication status	Published (in print/issue) - Nov 2001

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10.1078/1438-4639-00090

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title = "Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis",

abstract = "Abstract A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets.",

author = "C Millar and J Moore and C Lowery and K McCorry and J Dooley",

year = "2001",

month = nov,

doi = "10.1078/1438-4639-00090",

language = "English",

volume = "204",

pages = "191--194",

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}

Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis. / Millar, C; Moore, J; Lowery, C et al.
In: International Journal of Hygiene and Environmental Health, Vol. 204, No. 2-3, 11.2001, p. 191-194.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis

AU - Millar, C

AU - Moore, J

AU - Lowery, C

AU - McCorry, K

AU - Dooley, J

PY - 2001/11

Y1 - 2001/11

N2 - Abstract A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets.

AB - Abstract A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets.

U2 - 10.1078/1438-4639-00090

DO - 10.1078/1438-4639-00090

M3 - Article

SN - 1618-131X

VL - 204

SP - 191

EP - 194

JO - International Journal of Hygiene and Environmental Health

JF - International Journal of Hygiene and Environmental Health

IS - 2-3

ER -

Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis

Abstract

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