A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.
|Journal||International Journal of Hygiene and Environmental Health|
|Publication status||Published - Nov 2001|
Millar, C., Moore, J., Lowery, C., McCorry, K., & Dooley, J. (2001). Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis. International Journal of Hygiene and Environmental Health, 204(2-3), 191-194. https://doi.org/10.1078/1438-4639-00090