Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis

C Millar, J Moore, C Lowery, K McCorry, J Dooley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.
Original languageEnglish
Pages (from-to)191-194
JournalInternational Journal of Hygiene and Environmental Health
Volume204
Issue number2-3
DOIs
Publication statusPublished - Nov 2001

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