TY - JOUR
T1 - Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis
AU - Millar, C
AU - Moore, J
AU - Lowery, C
AU - McCorry, K
AU - Dooley, J
PY - 2001/11
Y1 - 2001/11
N2 - Abstract
A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets.
AB - Abstract
A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets.
U2 - 10.1078/1438-4639-00090
DO - 10.1078/1438-4639-00090
M3 - Article
SN - 1618-131X
VL - 204
SP - 191
EP - 194
JO - International Journal of Hygiene and Environmental Health
JF - International Journal of Hygiene and Environmental Health
IS - 2-3
ER -