Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis

C Millar, J Moore, C Lowery, K McCorry, J Dooley

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.
LanguageEnglish
Pages191-194
JournalInternational Journal of Hygiene and Environmental Health
Volume204
Issue number2-3
DOIs
Publication statusPublished - Nov 2001

Fingerprint

Cryptosporidium parvum
Oocysts
Disease Outbreaks
Furunculosis
Polymerase Chain Reaction
DNA
Alkalies

Cite this

@article{1f0e2568508b4e4a9aa22d6442e9d79a,
title = "Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis",
abstract = "A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.",
author = "C Millar and J Moore and C Lowery and K McCorry and J Dooley",
year = "2001",
month = "11",
doi = "10.1078/1438-4639-00090",
language = "English",
volume = "204",
pages = "191--194",
journal = "International Journal of Hygiene and Environmental Health",
issn = "1438-4639",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis

AU - Millar, C

AU - Moore, J

AU - Lowery, C

AU - McCorry, K

AU - Dooley, J

PY - 2001/11

Y1 - 2001/11

N2 - A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.

AB - A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.

U2 - 10.1078/1438-4639-00090

DO - 10.1078/1438-4639-00090

M3 - Article

VL - 204

SP - 191

EP - 194

JO - International Journal of Hygiene and Environmental Health

T2 - International Journal of Hygiene and Environmental Health

JF - International Journal of Hygiene and Environmental Health

SN - 1438-4639

IS - 2-3

ER -