Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction

Vinay Sharma; Rajveer Rajaura; Preetam Kumar Sharma; Rishabh Ronin Srivastava; Shyam Sundar Sharma; Kailash Agrawal

doi:10.1063/1.4946577

Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction

Vinay Sharma
, Rajveer Rajaura
, Preetam Kumar Sharma
, Rishabh Ronin Srivastava
, Shyam Sundar Sharma
, Kailash Agrawal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

An emerging area of research is improving the efficiency of the polymerase chain reaction (PCR) by using nanoparticles. With graphene nano-flakes showing promising results, in this paper we report the effect of Graphene-ZnO nanocomposites on Polymerase Chain reaction (PCR) efficiency. G-ZnO nanocomposites were efficiently synthesized via in situ chemical method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) image confirms the formation of nanocomposites. ZnO nanoparticles of size range ~20-30nm are uniformly attached on the graphene sheets. No amplification during PCR indicates inhibitory activity of G-ZnO nanocomposites which points the fingers at ZnO moiety of the G-ZnO composite for no amplification during our PCR reaction. Further work should concentrate on finding out the main inhibitory mechanism involved in inhibition of PCR using G-ZnO composites.

Original language	English
Title of host publication	International Conference on Condensed Matter and Applied Physics, ICC 2015
Subtitle of host publication	Proceeding of International Conference on Condensed Matter and Applied Physics
Editors	Manoj Singh Shekhawat, Sudhir Bhardwaj, Bhuvneshwer Suthar
Publisher	American Institute of Physics Inc.
Volume	1728
ISBN (Electronic)	9780735413757
DOIs	https://doi.org/10.1063/1.4946577
Publication status	Published (in print/issue) - 6 May 2016
Event	International Conference on Condensed Matter and Applied Physics, ICC 2015 - Bikaner, India Duration: 30 Oct 2015 → 31 Oct 2015

Conference

Conference	International Conference on Condensed Matter and Applied Physics, ICC 2015
Country/Territory	India
City	Bikaner
Period	30/10/15 → 31/10/15

Keywords

Graphene-ZnO
PCR
PCR Inhibition.
SEM
TEM

Access to Document

10.1063/1.4946577

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Sharma, V., Rajaura, R., Sharma, P. K., Srivastava, R. R., Sharma, S. S., & Agrawal, K. (2016). Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction. In M. S. Shekhawat, S. Bhardwaj, & B. Suthar (Eds.), International Conference on Condensed Matter and Applied Physics, ICC 2015: Proceeding of International Conference on Condensed Matter and Applied Physics (Vol. 1728). Article 020526 American Institute of Physics Inc.. https://doi.org/10.1063/1.4946577

Sharma, Vinay ; Rajaura, Rajveer ; Sharma, Preetam Kumar et al. / Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction. International Conference on Condensed Matter and Applied Physics, ICC 2015: Proceeding of International Conference on Condensed Matter and Applied Physics. editor / Manoj Singh Shekhawat ; Sudhir Bhardwaj ; Bhuvneshwer Suthar. Vol. 1728 American Institute of Physics Inc., 2016.

@inproceedings{cf1415dec6c14459858ef7ef516a8d8f,

title = "Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction",

abstract = "An emerging area of research is improving the efficiency of the polymerase chain reaction (PCR) by using nanoparticles. With graphene nano-flakes showing promising results, in this paper we report the effect of Graphene-ZnO nanocomposites on Polymerase Chain reaction (PCR) efficiency. G-ZnO nanocomposites were efficiently synthesized via in situ chemical method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) image confirms the formation of nanocomposites. ZnO nanoparticles of size range \textasciitilde{}20-30nm are uniformly attached on the graphene sheets. No amplification during PCR indicates inhibitory activity of G-ZnO nanocomposites which points the fingers at ZnO moiety of the G-ZnO composite for no amplification during our PCR reaction. Further work should concentrate on finding out the main inhibitory mechanism involved in inhibition of PCR using G-ZnO composites.",

keywords = "Graphene-ZnO, PCR, PCR Inhibition., SEM, TEM",

author = "Vinay Sharma and Rajveer Rajaura and Sharma, \{Preetam Kumar\} and Srivastava, \{Rishabh Ronin\} and Sharma, \{Shyam Sundar\} and Kailash Agrawal",

year = "2016",

month = may,

day = "6",

doi = "10.1063/1.4946577",

language = "English",

volume = "1728",

editor = "Shekhawat, \{Manoj Singh\} and Sudhir Bhardwaj and Bhuvneshwer Suthar",

booktitle = "International Conference on Condensed Matter and Applied Physics, ICC 2015",

publisher = "American Institute of Physics Inc.",

note = "International Conference on Condensed Matter and Applied Physics, ICC 2015 ; Conference date: 30-10-2015 Through 31-10-2015",

}

Sharma, V, Rajaura, R, Sharma, PK, Srivastava, RR, Sharma, SS & Agrawal, K 2016, Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction. in MS Shekhawat, S Bhardwaj & B Suthar (eds), International Conference on Condensed Matter and Applied Physics, ICC 2015: Proceeding of International Conference on Condensed Matter and Applied Physics. vol. 1728, 020526, American Institute of Physics Inc., International Conference on Condensed Matter and Applied Physics, ICC 2015, Bikaner, India, 30/10/15. https://doi.org/10.1063/1.4946577

Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction. / Sharma, Vinay; Rajaura, Rajveer; Sharma, Preetam Kumar et al.
International Conference on Condensed Matter and Applied Physics, ICC 2015: Proceeding of International Conference on Condensed Matter and Applied Physics. ed. / Manoj Singh Shekhawat; Sudhir Bhardwaj; Bhuvneshwer Suthar. Vol. 1728 American Institute of Physics Inc., 2016. 020526.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction

AU - Sharma, Vinay

AU - Rajaura, Rajveer

AU - Sharma, Preetam Kumar

AU - Srivastava, Rishabh Ronin

AU - Sharma, Shyam Sundar

AU - Agrawal, Kailash

PY - 2016/5/6

Y1 - 2016/5/6

N2 - An emerging area of research is improving the efficiency of the polymerase chain reaction (PCR) by using nanoparticles. With graphene nano-flakes showing promising results, in this paper we report the effect of Graphene-ZnO nanocomposites on Polymerase Chain reaction (PCR) efficiency. G-ZnO nanocomposites were efficiently synthesized via in situ chemical method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) image confirms the formation of nanocomposites. ZnO nanoparticles of size range ~20-30nm are uniformly attached on the graphene sheets. No amplification during PCR indicates inhibitory activity of G-ZnO nanocomposites which points the fingers at ZnO moiety of the G-ZnO composite for no amplification during our PCR reaction. Further work should concentrate on finding out the main inhibitory mechanism involved in inhibition of PCR using G-ZnO composites.

AB - An emerging area of research is improving the efficiency of the polymerase chain reaction (PCR) by using nanoparticles. With graphene nano-flakes showing promising results, in this paper we report the effect of Graphene-ZnO nanocomposites on Polymerase Chain reaction (PCR) efficiency. G-ZnO nanocomposites were efficiently synthesized via in situ chemical method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) image confirms the formation of nanocomposites. ZnO nanoparticles of size range ~20-30nm are uniformly attached on the graphene sheets. No amplification during PCR indicates inhibitory activity of G-ZnO nanocomposites which points the fingers at ZnO moiety of the G-ZnO composite for no amplification during our PCR reaction. Further work should concentrate on finding out the main inhibitory mechanism involved in inhibition of PCR using G-ZnO composites.

KW - Graphene-ZnO

KW - PCR

KW - PCR Inhibition.

KW - SEM

KW - TEM

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U2 - 10.1063/1.4946577

DO - 10.1063/1.4946577

M3 - Conference contribution

AN - SCOPUS:84984576627

VL - 1728

BT - International Conference on Condensed Matter and Applied Physics, ICC 2015

A2 - Shekhawat, Manoj Singh

A2 - Bhardwaj, Sudhir

A2 - Suthar, Bhuvneshwer

PB - American Institute of Physics Inc.

T2 - International Conference on Condensed Matter and Applied Physics, ICC 2015

Y2 - 30 October 2015 through 31 October 2015

ER -

Sharma V, Rajaura R, Sharma PK, Srivastava RR, Sharma SS, Agrawal K. Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction. In Shekhawat MS, Bhardwaj S, Suthar B, editors, International Conference on Condensed Matter and Applied Physics, ICC 2015: Proceeding of International Conference on Condensed Matter and Applied Physics. Vol. 1728. American Institute of Physics Inc. 2016. 020526 doi: 10.1063/1.4946577

Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction

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