An emerging area of research is improving the efficiency of the polymerase chain reaction (PCR) by using nanoparticles. With graphene nano-flakes showing promising results, in this paper we report the effect of Graphene-ZnO nanocomposites on Polymerase Chain reaction (PCR) efficiency. G-ZnO nanocomposites were efficiently synthesized via in situ chemical method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) image confirms the formation of nanocomposites. ZnO nanoparticles of size range ~20-30nm are uniformly attached on the graphene sheets. No amplification during PCR indicates inhibitory activity of G-ZnO nanocomposites which points the fingers at ZnO moiety of the G-ZnO composite for no amplification during our PCR reaction. Further work should concentrate on finding out the main inhibitory mechanism involved in inhibition of PCR using G-ZnO composites.
|Title of host publication||International Conference on Condensed Matter and Applied Physics, ICC 2015|
|Subtitle of host publication||Proceeding of International Conference on Condensed Matter and Applied Physics|
|Editors||Manoj Singh Shekhawat, Sudhir Bhardwaj, Bhuvneshwer Suthar|
|Publisher||American Institute of Physics Inc.|
|Publication status||Published (in print/issue) - 6 May 2016|
|Event||International Conference on Condensed Matter and Applied Physics, ICC 2015 - Bikaner, India|
Duration: 30 Oct 2015 → 31 Oct 2015
|Conference||International Conference on Condensed Matter and Applied Physics, ICC 2015|
|Period||30/10/15 → 31/10/15|
- PCR Inhibition.