Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction

Vinay Sharma, Rajveer Rajaura, Preetam Kumar Sharma, Rishabh Ronin Srivastava, Shyam Sundar Sharma, Kailash Agrawal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

An emerging area of research is improving the efficiency of the polymerase chain reaction (PCR) by using nanoparticles. With graphene nano-flakes showing promising results, in this paper we report the effect of Graphene-ZnO nanocomposites on Polymerase Chain reaction (PCR) efficiency. G-ZnO nanocomposites were efficiently synthesized via in situ chemical method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) image confirms the formation of nanocomposites. ZnO nanoparticles of size range ~20-30nm are uniformly attached on the graphene sheets. No amplification during PCR indicates inhibitory activity of G-ZnO nanocomposites which points the fingers at ZnO moiety of the G-ZnO composite for no amplification during our PCR reaction. Further work should concentrate on finding out the main inhibitory mechanism involved in inhibition of PCR using G-ZnO composites.

Original languageEnglish
Title of host publicationInternational Conference on Condensed Matter and Applied Physics, ICC 2015
Subtitle of host publicationProceeding of International Conference on Condensed Matter and Applied Physics
EditorsManoj Singh Shekhawat, Sudhir Bhardwaj, Bhuvneshwer Suthar
PublisherAmerican Institute of Physics Inc.
Volume1728
ISBN (Electronic)9780735413757
DOIs
Publication statusPublished - 6 May 2016
EventInternational Conference on Condensed Matter and Applied Physics, ICC 2015 - Bikaner, India
Duration: 30 Oct 201531 Oct 2015

Conference

ConferenceInternational Conference on Condensed Matter and Applied Physics, ICC 2015
CountryIndia
CityBikaner
Period30/10/1531/10/15

Keywords

  • Graphene-ZnO
  • PCR
  • PCR Inhibition.
  • SEM
  • TEM

Fingerprint Dive into the research topics of 'Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction'. Together they form a unique fingerprint.

  • Cite this

    Sharma, V., Rajaura, R., Sharma, P. K., Srivastava, R. R., Sharma, S. S., & Agrawal, K. (2016). Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction. In M. S. Shekhawat, S. Bhardwaj, & B. Suthar (Eds.), International Conference on Condensed Matter and Applied Physics, ICC 2015: Proceeding of International Conference on Condensed Matter and Applied Physics (Vol. 1728). [020526] American Institute of Physics Inc.. https://doi.org/10.1063/1.4946577