Study of the white-rot fungal degradation of selected phthalocyanine dyes by capillary electrophoresis and liquid chromatography

A Conneely, Franklin Smyth, Geoffrey McMullan

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    The phthalocyanine dyes, Remazol Turquoise Blue G133, Everzol Turquoise Blue and Heligon Blue S4 are found to be biosorbed by Phanerochaete chrysosporium (white-rot fungi) and also metabolised by its ligninolytic extracellular enzymes resulting in dye decolourisation, formation of free copper ions and organic metabolites with ultimate extensive phthalocyanine ring break-down. It is believed that the ligninolytic extracellular enzyme laccase is involved in the early production of a metabolite M-8 which involves break-up of the conjugated phthalocyanine ring structure but which retains multi-negative charge. Another ligninolytic extracellular enzyme, manganese peroxidase, is believed to be involved in the release of Cu2+ from the phthalocyanine structure to give a non-copper-containing phthalocyanine metabolite M-1 with a slightly longer migration time than the parent dye and absorption at 666 nm. The phthalocyanine ring structure is also broken up by metabolic processes that involve desulphonation and oxidation to give phthalimide (M-3) and an unidentified electroactive metabolite M-2. Other minor, unidentified metabolites are observed using capillary electrophoresis and liquid chromatography. (C) 2002 Elsevier Science B.V. All rights reserved.
    LanguageEnglish
    Pages259-270
    JournalAnalytica Chimica Acta
    Volume451
    Issue number2
    Publication statusPublished - Jan 2002

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    Capillary electrophoresis
    Liquid chromatography
    Coloring Agents
    Degradation
    Metabolites
    manganese peroxidase
    Enzymes
    Laccase
    Fungi
    phthalocyanine
    Copper
    Ions
    Oxidation

    Cite this

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    title = "Study of the white-rot fungal degradation of selected phthalocyanine dyes by capillary electrophoresis and liquid chromatography",
    abstract = "The phthalocyanine dyes, Remazol Turquoise Blue G133, Everzol Turquoise Blue and Heligon Blue S4 are found to be biosorbed by Phanerochaete chrysosporium (white-rot fungi) and also metabolised by its ligninolytic extracellular enzymes resulting in dye decolourisation, formation of free copper ions and organic metabolites with ultimate extensive phthalocyanine ring break-down. It is believed that the ligninolytic extracellular enzyme laccase is involved in the early production of a metabolite M-8 which involves break-up of the conjugated phthalocyanine ring structure but which retains multi-negative charge. Another ligninolytic extracellular enzyme, manganese peroxidase, is believed to be involved in the release of Cu2+ from the phthalocyanine structure to give a non-copper-containing phthalocyanine metabolite M-1 with a slightly longer migration time than the parent dye and absorption at 666 nm. The phthalocyanine ring structure is also broken up by metabolic processes that involve desulphonation and oxidation to give phthalimide (M-3) and an unidentified electroactive metabolite M-2. Other minor, unidentified metabolites are observed using capillary electrophoresis and liquid chromatography. (C) 2002 Elsevier Science B.V. All rights reserved.",
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    language = "English",
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    pages = "259--270",
    journal = "Analytica Chimica Acta",
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    Study of the white-rot fungal degradation of selected phthalocyanine dyes by capillary electrophoresis and liquid chromatography. / Conneely, A; Smyth, Franklin; McMullan, Geoffrey.

    In: Analytica Chimica Acta, Vol. 451, No. 2, 01.2002, p. 259-270.

    Research output: Contribution to journalArticle

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    AU - Smyth, Franklin

    AU - McMullan, Geoffrey

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    N2 - The phthalocyanine dyes, Remazol Turquoise Blue G133, Everzol Turquoise Blue and Heligon Blue S4 are found to be biosorbed by Phanerochaete chrysosporium (white-rot fungi) and also metabolised by its ligninolytic extracellular enzymes resulting in dye decolourisation, formation of free copper ions and organic metabolites with ultimate extensive phthalocyanine ring break-down. It is believed that the ligninolytic extracellular enzyme laccase is involved in the early production of a metabolite M-8 which involves break-up of the conjugated phthalocyanine ring structure but which retains multi-negative charge. Another ligninolytic extracellular enzyme, manganese peroxidase, is believed to be involved in the release of Cu2+ from the phthalocyanine structure to give a non-copper-containing phthalocyanine metabolite M-1 with a slightly longer migration time than the parent dye and absorption at 666 nm. The phthalocyanine ring structure is also broken up by metabolic processes that involve desulphonation and oxidation to give phthalimide (M-3) and an unidentified electroactive metabolite M-2. Other minor, unidentified metabolites are observed using capillary electrophoresis and liquid chromatography. (C) 2002 Elsevier Science B.V. All rights reserved.

    AB - The phthalocyanine dyes, Remazol Turquoise Blue G133, Everzol Turquoise Blue and Heligon Blue S4 are found to be biosorbed by Phanerochaete chrysosporium (white-rot fungi) and also metabolised by its ligninolytic extracellular enzymes resulting in dye decolourisation, formation of free copper ions and organic metabolites with ultimate extensive phthalocyanine ring break-down. It is believed that the ligninolytic extracellular enzyme laccase is involved in the early production of a metabolite M-8 which involves break-up of the conjugated phthalocyanine ring structure but which retains multi-negative charge. Another ligninolytic extracellular enzyme, manganese peroxidase, is believed to be involved in the release of Cu2+ from the phthalocyanine structure to give a non-copper-containing phthalocyanine metabolite M-1 with a slightly longer migration time than the parent dye and absorption at 666 nm. The phthalocyanine ring structure is also broken up by metabolic processes that involve desulphonation and oxidation to give phthalimide (M-3) and an unidentified electroactive metabolite M-2. Other minor, unidentified metabolites are observed using capillary electrophoresis and liquid chromatography. (C) 2002 Elsevier Science B.V. All rights reserved.

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