Study of the capillary zone electrophoretic behaviour of selected drugs, and its comparison with other analytical techniques for their formulation assay

G McGrath, Stephen McClean, E O'Kane, Franklin Smyth, F Tagliaro

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Capillary zone electrophoresis (CZE) was used to study the migration behaviour of selected 1,4-benzodiazepines and metabolites over the pH range 2-12, exhibiting the ability to determine pK(a) values using this technique. The selectivity of capillary electrophoresis was then demonstrated for the separation of four benzodiazepines using capillary zone electrophoresis with 20 mM citric acid + 15% methanol, and micellar electrokinetic capillary chromatography with 75 mM sodium dodecyl sulphate in 6 mM sodium tetraborate-12 mM disodium hydrogen phosphate + 5% MeOH, and compared with other topical analytical techniques in terms of retention times, capacity factors and efficiencies. Capillary zone electrophoresis was also applied to the assay of a variety of pharmaceutical formulations which contain 1,4-benzodiazepines, omeprazole and metronidazole, and was compared with alternative analytical techniques such as reversed-phase high-performance liquid chromatography, capillary gas chromatography and automated differential pulse polarography. Limits of detection of CZE and the alternative techniques are also compared for these molecules.
LanguageEnglish
Pages237-247
JournalJOURNAL OF CHROMATOGRAPHY A
Volume735
Issue number1-2
Publication statusPublished - May 1996

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Electrophoresis
Assays
Pharmaceutical Preparations
Polarographic analysis
Capillary electrophoresis
Omeprazole
Metronidazole
High performance liquid chromatography
Metabolites
Chromatography
Benzodiazepines
Citric Acid
Sodium Dodecyl Sulfate
Gas chromatography
Methanol
Molecules
Bz-423

Cite this

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abstract = "Capillary zone electrophoresis (CZE) was used to study the migration behaviour of selected 1,4-benzodiazepines and metabolites over the pH range 2-12, exhibiting the ability to determine pK(a) values using this technique. The selectivity of capillary electrophoresis was then demonstrated for the separation of four benzodiazepines using capillary zone electrophoresis with 20 mM citric acid + 15{\%} methanol, and micellar electrokinetic capillary chromatography with 75 mM sodium dodecyl sulphate in 6 mM sodium tetraborate-12 mM disodium hydrogen phosphate + 5{\%} MeOH, and compared with other topical analytical techniques in terms of retention times, capacity factors and efficiencies. Capillary zone electrophoresis was also applied to the assay of a variety of pharmaceutical formulations which contain 1,4-benzodiazepines, omeprazole and metronidazole, and was compared with alternative analytical techniques such as reversed-phase high-performance liquid chromatography, capillary gas chromatography and automated differential pulse polarography. Limits of detection of CZE and the alternative techniques are also compared for these molecules.",
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Study of the capillary zone electrophoretic behaviour of selected drugs, and its comparison with other analytical techniques for their formulation assay. / McGrath, G; McClean, Stephen; O'Kane, E; Smyth, Franklin; Tagliaro, F.

In: JOURNAL OF CHROMATOGRAPHY A, Vol. 735, No. 1-2, 05.1996, p. 237-247.

Research output: Contribution to journalArticle

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AU - McGrath, G

AU - McClean, Stephen

AU - O'Kane, E

AU - Smyth, Franklin

AU - Tagliaro, F

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AB - Capillary zone electrophoresis (CZE) was used to study the migration behaviour of selected 1,4-benzodiazepines and metabolites over the pH range 2-12, exhibiting the ability to determine pK(a) values using this technique. The selectivity of capillary electrophoresis was then demonstrated for the separation of four benzodiazepines using capillary zone electrophoresis with 20 mM citric acid + 15% methanol, and micellar electrokinetic capillary chromatography with 75 mM sodium dodecyl sulphate in 6 mM sodium tetraborate-12 mM disodium hydrogen phosphate + 5% MeOH, and compared with other topical analytical techniques in terms of retention times, capacity factors and efficiencies. Capillary zone electrophoresis was also applied to the assay of a variety of pharmaceutical formulations which contain 1,4-benzodiazepines, omeprazole and metronidazole, and was compared with alternative analytical techniques such as reversed-phase high-performance liquid chromatography, capillary gas chromatography and automated differential pulse polarography. Limits of detection of CZE and the alternative techniques are also compared for these molecules.

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