Abstract
Language | English |
---|---|
Pages | 221-230 |
Journal | Journal of General and Applied Microbiology |
Volume | 31 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1985 |
Fingerprint
Cite this
}
Study of immobilized yeast, Saccharomyces cerevisiae, using flow microcalorimetry. / Owusu, Richard; Finch, Arthur.
In: Journal of General and Applied Microbiology, Vol. 31, No. 3, 1985, p. 221-230.Research output: Contribution to journal › Article
TY - JOUR
T1 - Study of immobilized yeast, Saccharomyces cerevisiae, using flow microcalorimetry.
AU - Owusu, Richard
AU - Finch, Arthur
PY - 1985
Y1 - 1985
N2 - When immobilized yeast was exposed to nutrients, the resulting heat effect (dQ/dt; J sec-1) increased exponentially with a doubling time (t2) of 2.2±0.3hr. The half life (dQ/dt) under non-growing conditions with sucrose as substrate was 84hr. The kinetics of the transformation of a series of sugars were characterised. The Michaelis constant (Km) and maximal heat effect, (dQ/dt)max, were determined using two common enzyme kinetics linearization plots. The shapes of the Eadie plots for some sugars are discussed in terms of currently proposed mechanisms of their uptake
AB - When immobilized yeast was exposed to nutrients, the resulting heat effect (dQ/dt; J sec-1) increased exponentially with a doubling time (t2) of 2.2±0.3hr. The half life (dQ/dt) under non-growing conditions with sucrose as substrate was 84hr. The kinetics of the transformation of a series of sugars were characterised. The Michaelis constant (Km) and maximal heat effect, (dQ/dt)max, were determined using two common enzyme kinetics linearization plots. The shapes of the Eadie plots for some sugars are discussed in terms of currently proposed mechanisms of their uptake
U2 - 10.2323/jgam.31.221
DO - 10.2323/jgam.31.221
M3 - Article
VL - 31
SP - 221
EP - 230
JO - Journal of General and Applied Microbiology
T2 - Journal of General and Applied Microbiology
JF - Journal of General and Applied Microbiology
SN - 0022-1260
IS - 3
ER -