STUDIES ON THE GROWTH OF A THERMOTOLERANT YEAST-STRAIN, KLUYVEROMYCES-MARXIANUS IMB3, ON SUCROSE CONTAINING MEDIA

M Fleming, N Barron, L McHale, Roger Marchant, Anthony McHale

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Abstract

A thermotolerant alcohol-producing yeast strain, Kluyveromyces marxianus IMB3 was shown to grow on sucrose (10% [w/v]) containing media at 45-degrees-C. Under such conditions the organism reached stationary phase within 20 hours and yielded ethanol concentrations in the region of 33g/L. During growth on sucrose containing media the organism was found to produce a cell- associated activity capable of hydrolysing sucrose. This activity was shown to have a Km of 5.0mM when sucrose was used as the substrate. In addition the enzyme was shown to have a pH optimum of 5.0 and a temperature optimum of 50-55-degrees-C and under those conditions the enzyme was shown to be relatively thermostable.
LanguageEnglish
Pages1195-1198
JournalBiotechnology Letters
Volume15
Issue number12
Publication statusPublished - Dec 1993

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Kluyveromyces marxianus
sucrose
yeasts
organisms
enzymes
alcohols
ethanol
temperature
cells

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title = "STUDIES ON THE GROWTH OF A THERMOTOLERANT YEAST-STRAIN, KLUYVEROMYCES-MARXIANUS IMB3, ON SUCROSE CONTAINING MEDIA",
abstract = "A thermotolerant alcohol-producing yeast strain, Kluyveromyces marxianus IMB3 was shown to grow on sucrose (10{\%} [w/v]) containing media at 45-degrees-C. Under such conditions the organism reached stationary phase within 20 hours and yielded ethanol concentrations in the region of 33g/L. During growth on sucrose containing media the organism was found to produce a cell- associated activity capable of hydrolysing sucrose. This activity was shown to have a Km of 5.0mM when sucrose was used as the substrate. In addition the enzyme was shown to have a pH optimum of 5.0 and a temperature optimum of 50-55-degrees-C and under those conditions the enzyme was shown to be relatively thermostable.",
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STUDIES ON THE GROWTH OF A THERMOTOLERANT YEAST-STRAIN, KLUYVEROMYCES-MARXIANUS IMB3, ON SUCROSE CONTAINING MEDIA. / Fleming, M; Barron, N; McHale, L; Marchant, Roger; McHale, Anthony.

In: Biotechnology Letters, Vol. 15, No. 12, 12.1993, p. 1195-1198.

Research output: Contribution to journalArticle

TY - JOUR

T1 - STUDIES ON THE GROWTH OF A THERMOTOLERANT YEAST-STRAIN, KLUYVEROMYCES-MARXIANUS IMB3, ON SUCROSE CONTAINING MEDIA

AU - Fleming, M

AU - Barron, N

AU - McHale, L

AU - Marchant, Roger

AU - McHale, Anthony

PY - 1993/12

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N2 - A thermotolerant alcohol-producing yeast strain, Kluyveromyces marxianus IMB3 was shown to grow on sucrose (10% [w/v]) containing media at 45-degrees-C. Under such conditions the organism reached stationary phase within 20 hours and yielded ethanol concentrations in the region of 33g/L. During growth on sucrose containing media the organism was found to produce a cell- associated activity capable of hydrolysing sucrose. This activity was shown to have a Km of 5.0mM when sucrose was used as the substrate. In addition the enzyme was shown to have a pH optimum of 5.0 and a temperature optimum of 50-55-degrees-C and under those conditions the enzyme was shown to be relatively thermostable.

AB - A thermotolerant alcohol-producing yeast strain, Kluyveromyces marxianus IMB3 was shown to grow on sucrose (10% [w/v]) containing media at 45-degrees-C. Under such conditions the organism reached stationary phase within 20 hours and yielded ethanol concentrations in the region of 33g/L. During growth on sucrose containing media the organism was found to produce a cell- associated activity capable of hydrolysing sucrose. This activity was shown to have a Km of 5.0mM when sucrose was used as the substrate. In addition the enzyme was shown to have a pH optimum of 5.0 and a temperature optimum of 50-55-degrees-C and under those conditions the enzyme was shown to be relatively thermostable.

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JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

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ER -