STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS

A IRHUMA, J GALLAGHER, TJ HACKETT, AP MCHALE

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Abstract

Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.
LanguageEnglish
Pages1-5
JournalBIOCHIMICA ET BIOPHYSICA ACTA
Volume1074
Issue number1
DOIs
Publication statusPublished - May 1991

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Streptomyces hygroscopicus
chitin
Actinobacteria
culture filtrates
chitinase
organisms

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IRHUMA, A ; GALLAGHER, J ; HACKETT, TJ ; MCHALE, AP. / STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS. In: BIOCHIMICA ET BIOPHYSICA ACTA. 1991 ; Vol. 1074, No. 1. pp. 1-5.
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abstract = "Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.",
author = "A IRHUMA and J GALLAGHER and TJ HACKETT and AP MCHALE",
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STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS. / IRHUMA, A; GALLAGHER, J; HACKETT, TJ; MCHALE, AP.

In: BIOCHIMICA ET BIOPHYSICA ACTA, Vol. 1074, No. 1, 05.1991, p. 1-5.

Research output: Contribution to journalArticle

TY - JOUR

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AB - Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.

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