TY - JOUR
T1 - STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS
AU - IRHUMA, A
AU - GALLAGHER, J
AU - HACKETT, TJ
AU - MCHALE, AP
PY - 1991/5
Y1 - 1991/5
N2 - Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.
AB - Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.
U2 - 10.1016/0304-4165(91)90029-G
DO - 10.1016/0304-4165(91)90029-G
M3 - Article
VL - 1074
SP - 1
EP - 5
JO - BIOCHIMICA ET BIOPHYSICA ACTA
JF - BIOCHIMICA ET BIOPHYSICA ACTA
IS - 1
ER -