Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.
IRHUMA, A., GALLAGHER, J., HACKETT, TJ., & MCHALE, AP. (1991). STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS. BIOCHIMICA ET BIOPHYSICA ACTA, 1074(1), 1-5. https://doi.org/10.1016/0304-4165(91)90029-G