STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS

A IRHUMA, J GALLAGHER, TJ HACKETT, AP MCHALE

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11 Citations (Scopus)

Abstract

Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.
Original languageEnglish
Pages (from-to)1-5
JournalBIOCHIMICA ET BIOPHYSICA ACTA
Volume1074
Issue number1
DOIs
Publication statusPublished - May 1991

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