Structure, antihyperglycemic activity and cellular actions of a novel diglycated human insulin

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Abstract

Human insulin was glycated under hyperglycemic reducing conditions and a novel diglycated form (M-r 6135.1 Da) was purified by RP-HPLC. Endoproteinase Glu-C digestion combined with mass spectrometry and automated Edman degradation localized glycation to Gly(1) and Phe(1) of the insulin A- and B-chains, respectively. Intraperitoneal (i.p.) administration of diglycated insulin to mice alone or in combination with glucose (7 nmol/kg) resulted in a 43-61% and 11-34% reduction in glucose lowering activity, respectively, compared with native insulin. Consistent with these findings, diglycated insulin (10(-9) to 10(-7) mol/liter) was 22-38% less effective (P <0.001) than native insulin in stimulating glucose uptake, glucose oxidation and glycogen production in isolated mouse abdominal muscle. (C) 2000 Elsevier Science Inc. All rights reserved.
Original languageEnglish
Pages (from-to)1519-1526
JournalPeptides
Volume21
Issue number10
Publication statusPublished - Oct 2000

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