Structural changes in cellular membranes induced by ionic liquids: From model to bacterial membranes

G. Bhattacharya, R. P. Giri, A. Dubey, S. Mitra, R. Priyadarshini, A. Gupta, M. K. Mukhopadhyay, Sajal Kumar Ghosh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ionic liquids (ILs) have generated considerable attention recently because of their cytotoxicity and application as antibiotics. However, the mechanism of how they damage cell membranes is not currently well understood. In this paper, the antibacterial activities of two imidazolium-based ILs, namely 1-butyl- 3-methylimidazolium tetrafluroborate ([BMIM][BF4]) and 1-ethyl- 3-methylimidazolium tetrafluroborate ([EMIM][BF4]) have been investigated. The activity of [BMIM][BF4] on gram negative bacteria E. coli is observed to be stronger compared with the short chained [EMIM][BF4]. To explain this observation, the effects of these ILs on the self-assembled structures of model cellular membranes have been investigated. The in-plane elasticity of a monolayer formed at air-water interface by 1,2-dipalmitoyl- sn-glycero- 3-phosphocholine (DPPC) lipids was reduced in the presence of the ILs. The x-ray reflectivity studies on polymer supported lipid bilayer have shown the bilayer to shrink and correspondingly exhibit an increase in electron density. The presence of a certain mol% of negatively charged lipid, 1,2-dipalmitoyl-rac-glycero-3-phospho-L-serine (DPPS), in DPPC mono- and bi-layers enhances the effect considerably.
LanguageEnglish
Pages1-10
JournalCHEMISTRY AND PHYSICS OF LIPIDS
Volume215
DOIs
Publication statusPublished - Sep 2018

Keywords

  • Cellular membrane
  • Ionic liquid
  • Lipid monolayer
  • Lipid bilayer
  • X-ray reflectivity

Cite this

Bhattacharya, G. ; Giri, R. P. ; Dubey, A. ; Mitra, S. ; Priyadarshini, R. ; Gupta, A. ; Mukhopadhyay, M. K. ; Ghosh, Sajal Kumar. / Structural changes in cellular membranes induced by ionic liquids: From model to bacterial membranes. In: CHEMISTRY AND PHYSICS OF LIPIDS. 2018 ; Vol. 215. pp. 1-10.
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abstract = "Ionic liquids (ILs) have generated considerable attention recently because of their cytotoxicity and application as antibiotics. However, the mechanism of how they damage cell membranes is not currently well understood. In this paper, the antibacterial activities of two imidazolium-based ILs, namely 1-butyl- 3-methylimidazolium tetrafluroborate ([BMIM][BF4]) and 1-ethyl- 3-methylimidazolium tetrafluroborate ([EMIM][BF4]) have been investigated. The activity of [BMIM][BF4] on gram negative bacteria E. coli is observed to be stronger compared with the short chained [EMIM][BF4]. To explain this observation, the effects of these ILs on the self-assembled structures of model cellular membranes have been investigated. The in-plane elasticity of a monolayer formed at air-water interface by 1,2-dipalmitoyl- sn-glycero- 3-phosphocholine (DPPC) lipids was reduced in the presence of the ILs. The x-ray reflectivity studies on polymer supported lipid bilayer have shown the bilayer to shrink and correspondingly exhibit an increase in electron density. The presence of a certain mol{\%} of negatively charged lipid, 1,2-dipalmitoyl-rac-glycero-3-phospho-L-serine (DPPS), in DPPC mono- and bi-layers enhances the effect considerably.",
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Structural changes in cellular membranes induced by ionic liquids: From model to bacterial membranes. / Bhattacharya, G.; Giri, R. P.; Dubey, A.; Mitra, S.; Priyadarshini, R.; Gupta, A.; Mukhopadhyay, M. K.; Ghosh, Sajal Kumar.

In: CHEMISTRY AND PHYSICS OF LIPIDS, Vol. 215, 09.2018, p. 1-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Structural changes in cellular membranes induced by ionic liquids: From model to bacterial membranes

AU - Bhattacharya, G.

AU - Giri, R. P.

AU - Dubey, A.

AU - Mitra, S.

AU - Priyadarshini, R.

AU - Gupta, A.

AU - Mukhopadhyay, M. K.

AU - Ghosh, Sajal Kumar

N1 - No Accepted Version

PY - 2018/9

Y1 - 2018/9

N2 - Ionic liquids (ILs) have generated considerable attention recently because of their cytotoxicity and application as antibiotics. However, the mechanism of how they damage cell membranes is not currently well understood. In this paper, the antibacterial activities of two imidazolium-based ILs, namely 1-butyl- 3-methylimidazolium tetrafluroborate ([BMIM][BF4]) and 1-ethyl- 3-methylimidazolium tetrafluroborate ([EMIM][BF4]) have been investigated. The activity of [BMIM][BF4] on gram negative bacteria E. coli is observed to be stronger compared with the short chained [EMIM][BF4]. To explain this observation, the effects of these ILs on the self-assembled structures of model cellular membranes have been investigated. The in-plane elasticity of a monolayer formed at air-water interface by 1,2-dipalmitoyl- sn-glycero- 3-phosphocholine (DPPC) lipids was reduced in the presence of the ILs. The x-ray reflectivity studies on polymer supported lipid bilayer have shown the bilayer to shrink and correspondingly exhibit an increase in electron density. The presence of a certain mol% of negatively charged lipid, 1,2-dipalmitoyl-rac-glycero-3-phospho-L-serine (DPPS), in DPPC mono- and bi-layers enhances the effect considerably.

AB - Ionic liquids (ILs) have generated considerable attention recently because of their cytotoxicity and application as antibiotics. However, the mechanism of how they damage cell membranes is not currently well understood. In this paper, the antibacterial activities of two imidazolium-based ILs, namely 1-butyl- 3-methylimidazolium tetrafluroborate ([BMIM][BF4]) and 1-ethyl- 3-methylimidazolium tetrafluroborate ([EMIM][BF4]) have been investigated. The activity of [BMIM][BF4] on gram negative bacteria E. coli is observed to be stronger compared with the short chained [EMIM][BF4]. To explain this observation, the effects of these ILs on the self-assembled structures of model cellular membranes have been investigated. The in-plane elasticity of a monolayer formed at air-water interface by 1,2-dipalmitoyl- sn-glycero- 3-phosphocholine (DPPC) lipids was reduced in the presence of the ILs. The x-ray reflectivity studies on polymer supported lipid bilayer have shown the bilayer to shrink and correspondingly exhibit an increase in electron density. The presence of a certain mol% of negatively charged lipid, 1,2-dipalmitoyl-rac-glycero-3-phospho-L-serine (DPPS), in DPPC mono- and bi-layers enhances the effect considerably.

KW - Cellular membrane

KW - Ionic liquid

KW - Lipid monolayer

KW - Lipid bilayer

KW - X-ray reflectivity

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T2 - CHEMISTRY AND PHYSICS OF LIPIDS

JF - CHEMISTRY AND PHYSICS OF LIPIDS

SN - 0009-3084

ER -