TY - JOUR
T1 - Structural basis for delta cell paracrine regulation in pancreatic islets
AU - Arrojo E Drigo, Rafael
AU - Jacob, Stefan
AU - García-prieto, Concha F.
AU - Zheng, Xiaofeng
AU - Fukuda, Masahiro
AU - Nhu, Hoa Tran Thi
AU - Stelmashenko, Olga
AU - Peçanha, Flavia Letícia Martins
AU - Rodriguez-diaz, Rayner
AU - Bushong, Eric
AU - Deerinck, Thomas
AU - Phan, Sebastien
AU - Ali, Yusuf
AU - Leibiger, Ingo
AU - Chua, Minni
AU - Boudier, Thomas
AU - Song, Sang-ho
AU - Graf, Martin
AU - Augustine, George J.
AU - Ellisman, Mark H.
AU - Berggren, Per-olof
PY - 2019/8/16
Y1 - 2019/8/16
N2 - Little is known about the role of islet delta cells in regulating blood glucose homeostasis in vivo. Delta cells are important paracrine regulators of beta cell and alpha cell secretory activity, however the structural basis underlying this regulation has yet to be determined. Most delta cells are elongated and have a well-defined cell soma and a filopodia-like structure. Using in vivo optogenetics and high-speed Ca2+ imaging, we show that these filopodia are dynamic structures that contain a secretory machinery, enabling the delta cell to reach a large number of beta cells within the islet. This provides for efficient regulation of beta cell activity and is modulated by endogenous IGF-1/VEGF-A signaling. In pre-diabetes, delta cells undergo morphological changes that may be a compensation to maintain paracrine regulation of the beta cell. Our data provides an integrated picture of how delta cells can modulate beta cell activity under physiological conditions.
AB - Little is known about the role of islet delta cells in regulating blood glucose homeostasis in vivo. Delta cells are important paracrine regulators of beta cell and alpha cell secretory activity, however the structural basis underlying this regulation has yet to be determined. Most delta cells are elongated and have a well-defined cell soma and a filopodia-like structure. Using in vivo optogenetics and high-speed Ca2+ imaging, we show that these filopodia are dynamic structures that contain a secretory machinery, enabling the delta cell to reach a large number of beta cells within the islet. This provides for efficient regulation of beta cell activity and is modulated by endogenous IGF-1/VEGF-A signaling. In pre-diabetes, delta cells undergo morphological changes that may be a compensation to maintain paracrine regulation of the beta cell. Our data provides an integrated picture of how delta cells can modulate beta cell activity under physiological conditions.
UR - http://www.nature.com/articles/s41467-019-11517-x
UR - https://pure.ulster.ac.uk/en/publications/structural-basis-for-delta-cell-paracrine-regulation-in-pancreati
U2 - 10.1038/s41467-019-11517-x
DO - 10.1038/s41467-019-11517-x
M3 - Article
C2 - 31420552
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3700 (2019)
ER -
