Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection

A Erdem, P Papakonstantinou, H Murphy, M McMullan, H Karadeniz, S Sharma

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Disposable graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (MWCNTs)-streptavidin (STR) conjugates were used for electrochemical monitoring of label-free DNA hybridization. The surface morphology of PGE electrode before and after hybridization was characterized by scanning electron microscopy. Electrochemical impedance spectroscopy was used to monitor each step of the construction of the DNA biosensor. The biosensor was demonstrated to have excellent selectivity, being able to differentiate complementary sequences from a noncomplementary ones and in addition select the target sequence of DNA from a mixture of other DNA without loss in current sensitivity.
LanguageEnglish
Pages611-617
JournalElectroanalysis
Volume22
Issue number6
DOIs
Publication statusPublished - Mar 2010

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Graphite electrodes
Carbon Nanotubes
Streptavidin
Labels
Carbon nanotubes
DNA
Prostaglandins E
Biosensors
Multiwalled carbon nanotubes (MWCN)
Electrochemical impedance spectroscopy
Surface morphology
Scanning electron microscopy
Electrodes
Monitoring

Cite this

Erdem, A ; Papakonstantinou, P ; Murphy, H ; McMullan, M ; Karadeniz, H ; Sharma, S. / Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection. In: Electroanalysis. 2010 ; Vol. 22, No. 6. pp. 611-617.
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Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection. / Erdem, A; Papakonstantinou, P; Murphy, H; McMullan, M; Karadeniz, H; Sharma, S.

In: Electroanalysis, Vol. 22, No. 6, 03.2010, p. 611-617.

Research output: Contribution to journalArticle

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