Stratification and Monitoring of Juvenile Idiopathic Arthritis Patientsby Synovial Proteome Analysis

David Gibson, Sorcha Finnegan, Grant Jordan, Caitriona Scaife, Simon Brockbank, Jim Curry, Catherine McAllister, Stephen Pennington, Michael Dunn, Madeleine Rooney

Research output: Contribution to journalArticle

Abstract

Juvenile idiopathic arthritis (JIA) comprises a poorly understood group of chronic, childhood onset,autoimmune diseases with variable clinical outcomes. We investigated whether profiling of the synovialfluid (SF) proteome by a fluorescent dye based, two-dimensional gel (DIGE) approach could distinguishpatients in whom inflammation extends to affect a large number of joints, early in the disease process.SF samples from 22 JIA patients were analyzed: 10 with oligoarticular arthritis, 5 extended oligoarticularand 7 polyarticular disease. SF samples were labeled with Cy dyes and separated by two-dimensionalelectrophoresis. Multivariate analyses were used to isolate a panel of proteins which distinguish patientsubgroups. Proteins were identified using MALDI-TOF mass spectrometry with expression furtherverified by Western immunoblotting and immunohistochemistry. Hierarchical clustering based on theexpression levels of a set of 40 proteins segregated the extended oligoarticular from the oligoarticularpatients (p <0.05). Expression patterns of the isolated protein panel have also been observed overtime, as disease spreads to multiple joints. The data indicates that synovial fluid proteome profilescould be used to stratify patients based on risk of disease extension. These protein profiles may alsoassist in monitoring therapeutic responses over time and help predict joint damage.
LanguageEnglish
Pages5601-5609
JournalJournal of Proteome Research
Volume8
Issue number12
Publication statusPublished - 22 Oct 2009

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Juvenile Arthritis
Proteome
Monitoring
Joints
Proteins
Synovial Fluid
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Fluorescent Dyes
Autoimmune Diseases
Arthritis
Mass spectrometry
Cluster Analysis
Mass Spectrometry
Coloring Agents
Multivariate Analysis
Western Blotting
Gels
Immunohistochemistry
Inflammation
Fluids

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Gibson, D., Finnegan, S., Jordan, G., Scaife, C., Brockbank, S., Curry, J., ... Rooney, M. (2009). Stratification and Monitoring of Juvenile Idiopathic Arthritis Patientsby Synovial Proteome Analysis. Journal of Proteome Research, 8(12), 5601-5609.
Gibson, David ; Finnegan, Sorcha ; Jordan, Grant ; Scaife, Caitriona ; Brockbank, Simon ; Curry, Jim ; McAllister, Catherine ; Pennington, Stephen ; Dunn, Michael ; Rooney, Madeleine. / Stratification and Monitoring of Juvenile Idiopathic Arthritis Patientsby Synovial Proteome Analysis. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 12. pp. 5601-5609.
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Gibson, D, Finnegan, S, Jordan, G, Scaife, C, Brockbank, S, Curry, J, McAllister, C, Pennington, S, Dunn, M & Rooney, M 2009, 'Stratification and Monitoring of Juvenile Idiopathic Arthritis Patientsby Synovial Proteome Analysis', Journal of Proteome Research, vol. 8, no. 12, pp. 5601-5609.

Stratification and Monitoring of Juvenile Idiopathic Arthritis Patientsby Synovial Proteome Analysis. / Gibson, David; Finnegan, Sorcha; Jordan, Grant; Scaife, Caitriona; Brockbank, Simon; Curry, Jim; McAllister, Catherine; Pennington, Stephen; Dunn, Michael; Rooney, Madeleine.

In: Journal of Proteome Research, Vol. 8, No. 12, 22.10.2009, p. 5601-5609.

Research output: Contribution to journalArticle

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Gibson D, Finnegan S, Jordan G, Scaife C, Brockbank S, Curry J et al. Stratification and Monitoring of Juvenile Idiopathic Arthritis Patientsby Synovial Proteome Analysis. Journal of Proteome Research. 2009 Oct 22;8(12):5601-5609.