SPR and AFM study of engineered biomolecule immobilisation techniques

Ian Craig; James A. McLaughlin

doi:10.1109/IEMBS.2006.260932

SPR and AFM study of engineered biomolecule immobilisation techniques

Ian Craig
, James A. McLaughlin

Engineering Research

Ulster University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

A comparative study into two novel and diverse schemes designed to improve immobilization of biomolecules for biosensing purposes is presented. In the first method a silicon rich matrix is created using PECVD. The second method involves creating nano-patterns on the sensor surface to create a large number of surface discontinuities to which the proteins will bind preferentially. The basic theory of SPR is provided to show the importance of the surface sensitive nature of this optical transduction technique. The present work suggests that both may prove both for SPR and other biosensing applications. Of the two schemes proposed, the results for nano-patterning seem to suggest that it is promoting better surface attachment of biomolecules. The results of SPR and AFM studies are presented that have shown that each of these schemes promotes improved binding of various proteins.

Original language	English
Title of host publication	28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Pages	6728-6731
Number of pages	4
DOIs	https://doi.org/10.1109/IEMBS.2006.260932
Publication status	Published (in print/issue) - 2006
Event	28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 - New York, NY, United States Duration: 30 Aug 2006 → 3 Sept 2006

Publication series

Name	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN (Print)	0589-1019

Conference

Conference	28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Country/Territory	United States
City	New York, NY
Period	30/08/06 → 3/09/06

Bibliographical note

Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.

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10.1109/IEMBS.2006.260932

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Craig, I., & McLaughlin, J. A. (2006). SPR and AFM study of engineered biomolecule immobilisation techniques. In 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 (pp. 6728-6731). Article 4030643 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings). https://doi.org/10.1109/IEMBS.2006.260932

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title = "SPR and AFM study of engineered biomolecule immobilisation techniques",

abstract = "A comparative study into two novel and diverse schemes designed to improve immobilization of biomolecules for biosensing purposes is presented. In the first method a silicon rich matrix is created using PECVD. The second method involves creating nano-patterns on the sensor surface to create a large number of surface discontinuities to which the proteins will bind preferentially. The basic theory of SPR is provided to show the importance of the surface sensitive nature of this optical transduction technique. The present work suggests that both may prove both for SPR and other biosensing applications. Of the two schemes proposed, the results for nano-patterning seem to suggest that it is promoting better surface attachment of biomolecules. The results of SPR and AFM studies are presented that have shown that each of these schemes promotes improved binding of various proteins.",

author = "Ian Craig and McLaughlin, \{James A.\}",

note = "Copyright: Copyright 2011 Elsevier B.V., All rights reserved.; 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 ; Conference date: 30-08-2006 Through 03-09-2006",

year = "2006",

doi = "10.1109/IEMBS.2006.260932",

language = "English",

isbn = "1424400325",

series = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

pages = "6728--6731",

booktitle = "28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06",

}

Craig, I & McLaughlin, JA 2006, SPR and AFM study of engineered biomolecule immobilisation techniques. in 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06., 4030643, Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, pp. 6728-6731, 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06, New York, NY, United States, 30/08/06. https://doi.org/10.1109/IEMBS.2006.260932

SPR and AFM study of engineered biomolecule immobilisation techniques. / Craig, Ian; McLaughlin, James A.
28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06. 2006. p. 6728-6731 4030643 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - McLaughlin, James A.

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AB - A comparative study into two novel and diverse schemes designed to improve immobilization of biomolecules for biosensing purposes is presented. In the first method a silicon rich matrix is created using PECVD. The second method involves creating nano-patterns on the sensor surface to create a large number of surface discontinuities to which the proteins will bind preferentially. The basic theory of SPR is provided to show the importance of the surface sensitive nature of this optical transduction technique. The present work suggests that both may prove both for SPR and other biosensing applications. Of the two schemes proposed, the results for nano-patterning seem to suggest that it is promoting better surface attachment of biomolecules. The results of SPR and AFM studies are presented that have shown that each of these schemes promotes improved binding of various proteins.

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DO - 10.1109/IEMBS.2006.260932

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SN - 9781424400324

T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

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BT - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06

T2 - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06

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ER -

SPR and AFM study of engineered biomolecule immobilisation techniques

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