SPR and AFM study of engineered biomolecule immobilisation techniques

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    1 Citation (Scopus)

    Abstract

    A comparative study into two novel and diverse schemes designed to improve immobilization of biomolecules for biosensing purposes is presented. In the first method a silicon rich matrix is created using PECVD. The second method involves creating nano-patterns on the sensor surface to create a large number of surface discontinuities to which the proteins will bind preferentially. The basic theory of SPR is provided to show the importance of the surface sensitive nature of this optical transduction technique. The present work suggests that both may prove both for SPR and other biosensing applications. Of the two schemes proposed, the results for nano-patterning seem to suggest that it is promoting better surface attachment of biomolecules. The results of SPR and AFM studies are presented that have shown that each of these schemes promotes improved binding of various proteins.

    Original languageEnglish
    Title of host publication28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
    Pages6728-6731
    Number of pages4
    DOIs
    Publication statusPublished - 2006
    Event28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 - New York, NY, United States
    Duration: 30 Aug 20063 Sep 2006

    Publication series

    NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
    ISSN (Print)0589-1019

    Conference

    Conference28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
    CountryUnited States
    CityNew York, NY
    Period30/08/063/09/06

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