Spatial Localization and Quantitation of Androgens in Mouse Testisby Mass Spectrometry Imaging

Research output: Contribution to journalArticle

21 Citations (Scopus)
8 Downloads (Pure)

Abstract

Androgens are essential for male development and reproductivefunction. They are transported to their site of action as blood-borneendocrine hormones but can also be produced within tissues to act inintracrine and paracrine fashions. Because of this, circulating concentrationsmay not accurately reflect the androgenic influence within specific tissuemicroenvironments. Mass spectrometry imaging permits regional analysis ofsmall molecular species directly from tissue surfaces. However, due to poorionization and localized ion suppression, steroid hormones are difficult todetect. Here, derivatization with Girard T reagent was used to charge-tagtestosterone and 5α-dihydrotestosterone allowing direct detection of thesesteroids in mouse testes, in both basal and maximally stimulated states, and inrat prostate. Limits of detection were ∼0.1 pg for testosterone. Exemplarydetection of endogenous steroids was achieved by matrix-assisted laserdesorption ionization and either Fourier transform ion cyclotron resonancedetection (at 150 μm spatial resolution) or quadrupole-time-of-flight detection (at 50 μm spatial resolution). Structuralconfirmation was achieved by collision induced fragmentation following liquid extraction surface analysis and electrosprayionization. This application broadens the scope for derivatization strategies on tissue surfaces to elucidate local endocrinesignaling in health and disease.
Original languageEnglish
Pages (from-to)10362-10367
Number of pages6
JournalAnalytical Chemistry
Volume88
DOIs
Publication statusPublished - 27 Sep 2016

Keywords

  • Androgens
  • Mass Spectrometry Imaging

Fingerprint Dive into the research topics of 'Spatial Localization and Quantitation of Androgens in Mouse Testisby Mass Spectrometry Imaging'. Together they form a unique fingerprint.

  • Cite this