Sophorolipids production by Candida bombicola ATCC 22214 and its potential application in microbial enhanced oil recovery

Abdulkadir E. Elshafie, Sanket J. Joshi, Yahya M. Al-Wahaibi, Ali S. Al-Bemani, Saif N. Al-Bahry, Duaa Al-Maqbali, Ibrahim Banat

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Biosurfactant production using Candida bombicola ATCC 22214, its characterization and potential applications in enhancing oil recovery were studied at laboratory scale. The seed media and the production media were standardized for optimal growth and biosurfactant production. The production media were tested with different carbon sources: glucose (2%w/v) and corn oil (10%v/v) added separately or concurrently. The samples were collected at 24 h interval up to 120 h and checked for growth (OD660), and biosurfactant production [surface tension (ST) and interfacial tension (IFT)]. The medium with both glucose and corn oil gave better biosurfactant production and reduced both ST and IFT to 28.56 + 0.42mN/m and 2.13 + 0.09mN/m, respectively within 72 h. The produced biosurfactant was quite stable at 13–15% salinity, pH range of 2–12, and at temperature up to 100°C. It also produced stable emulsions (%E24) with different hydrocarbons (pentane, hexane, heptane, tridecane, tetradecane, hexadecane, 1-methylnaphthalene, 2,2,4,4,6,8-heptamethylnonane, light and heavy crude oil). The produced biosurfactant was extracted using ethyl acetate and characterized as a mixture of sophorolipids (SPLs). The potential of SPLs in enhancing oil recovery was tested using core-flooding experiments under reservoir conditions, where additional 27.27% of residual oil (Sor) was recovered. This confirmed the potential of SPLs for applications in microbial enhanced oil recovery.
LanguageEnglish
JournalFrontiers in Microbiology
Volume6
DOIs
Publication statusPublished - 2015

Fingerprint

sophorolipids
biosurfactants
surface tension
oils
corn oil
hexadecane
heptane
pentane
glucose
hexane
emulsions
hydrocarbons
Starmerella bombicola
salinity
carbon
seeds

Keywords

  • biosurfactant
  • sophorolipids
  • Candida bombicola
  • microbial enhanced oil recovery
  • core-flood
  • experiment

Cite this

Elshafie, Abdulkadir E. ; Joshi, Sanket J. ; Al-Wahaibi, Yahya M. ; Al-Bemani, Ali S. ; Al-Bahry, Saif N. ; Al-Maqbali, Duaa ; Banat, Ibrahim. / Sophorolipids production by Candida bombicola ATCC 22214 and its potential application in microbial enhanced oil recovery. In: Frontiers in Microbiology. 2015 ; Vol. 6.
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title = "Sophorolipids production by Candida bombicola ATCC 22214 and its potential application in microbial enhanced oil recovery",
abstract = "Biosurfactant production using Candida bombicola ATCC 22214, its characterization and potential applications in enhancing oil recovery were studied at laboratory scale. The seed media and the production media were standardized for optimal growth and biosurfactant production. The production media were tested with different carbon sources: glucose (2{\%}w/v) and corn oil (10{\%}v/v) added separately or concurrently. The samples were collected at 24 h interval up to 120 h and checked for growth (OD660), and biosurfactant production [surface tension (ST) and interfacial tension (IFT)]. The medium with both glucose and corn oil gave better biosurfactant production and reduced both ST and IFT to 28.56 + 0.42mN/m and 2.13 + 0.09mN/m, respectively within 72 h. The produced biosurfactant was quite stable at 13–15{\%} salinity, pH range of 2–12, and at temperature up to 100°C. It also produced stable emulsions ({\%}E24) with different hydrocarbons (pentane, hexane, heptane, tridecane, tetradecane, hexadecane, 1-methylnaphthalene, 2,2,4,4,6,8-heptamethylnonane, light and heavy crude oil). The produced biosurfactant was extracted using ethyl acetate and characterized as a mixture of sophorolipids (SPLs). The potential of SPLs in enhancing oil recovery was tested using core-flooding experiments under reservoir conditions, where additional 27.27{\%} of residual oil (Sor) was recovered. This confirmed the potential of SPLs for applications in microbial enhanced oil recovery.",
keywords = "biosurfactant, sophorolipids, Candida bombicola, microbial enhanced oil recovery, core-flood, experiment",
author = "Elshafie, {Abdulkadir E.} and Joshi, {Sanket J.} and Al-Wahaibi, {Yahya M.} and Al-Bemani, {Ali S.} and Al-Bahry, {Saif N.} and Duaa Al-Maqbali and Ibrahim Banat",
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Sophorolipids production by Candida bombicola ATCC 22214 and its potential application in microbial enhanced oil recovery. / Elshafie, Abdulkadir E.; Joshi, Sanket J.; Al-Wahaibi, Yahya M.; Al-Bemani, Ali S.; Al-Bahry, Saif N.; Al-Maqbali, Duaa; Banat, Ibrahim.

In: Frontiers in Microbiology, Vol. 6, 2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sophorolipids production by Candida bombicola ATCC 22214 and its potential application in microbial enhanced oil recovery

AU - Elshafie, Abdulkadir E.

AU - Joshi, Sanket J.

AU - Al-Wahaibi, Yahya M.

AU - Al-Bemani, Ali S.

AU - Al-Bahry, Saif N.

AU - Al-Maqbali, Duaa

AU - Banat, Ibrahim

PY - 2015

Y1 - 2015

N2 - Biosurfactant production using Candida bombicola ATCC 22214, its characterization and potential applications in enhancing oil recovery were studied at laboratory scale. The seed media and the production media were standardized for optimal growth and biosurfactant production. The production media were tested with different carbon sources: glucose (2%w/v) and corn oil (10%v/v) added separately or concurrently. The samples were collected at 24 h interval up to 120 h and checked for growth (OD660), and biosurfactant production [surface tension (ST) and interfacial tension (IFT)]. The medium with both glucose and corn oil gave better biosurfactant production and reduced both ST and IFT to 28.56 + 0.42mN/m and 2.13 + 0.09mN/m, respectively within 72 h. The produced biosurfactant was quite stable at 13–15% salinity, pH range of 2–12, and at temperature up to 100°C. It also produced stable emulsions (%E24) with different hydrocarbons (pentane, hexane, heptane, tridecane, tetradecane, hexadecane, 1-methylnaphthalene, 2,2,4,4,6,8-heptamethylnonane, light and heavy crude oil). The produced biosurfactant was extracted using ethyl acetate and characterized as a mixture of sophorolipids (SPLs). The potential of SPLs in enhancing oil recovery was tested using core-flooding experiments under reservoir conditions, where additional 27.27% of residual oil (Sor) was recovered. This confirmed the potential of SPLs for applications in microbial enhanced oil recovery.

AB - Biosurfactant production using Candida bombicola ATCC 22214, its characterization and potential applications in enhancing oil recovery were studied at laboratory scale. The seed media and the production media were standardized for optimal growth and biosurfactant production. The production media were tested with different carbon sources: glucose (2%w/v) and corn oil (10%v/v) added separately or concurrently. The samples were collected at 24 h interval up to 120 h and checked for growth (OD660), and biosurfactant production [surface tension (ST) and interfacial tension (IFT)]. The medium with both glucose and corn oil gave better biosurfactant production and reduced both ST and IFT to 28.56 + 0.42mN/m and 2.13 + 0.09mN/m, respectively within 72 h. The produced biosurfactant was quite stable at 13–15% salinity, pH range of 2–12, and at temperature up to 100°C. It also produced stable emulsions (%E24) with different hydrocarbons (pentane, hexane, heptane, tridecane, tetradecane, hexadecane, 1-methylnaphthalene, 2,2,4,4,6,8-heptamethylnonane, light and heavy crude oil). The produced biosurfactant was extracted using ethyl acetate and characterized as a mixture of sophorolipids (SPLs). The potential of SPLs in enhancing oil recovery was tested using core-flooding experiments under reservoir conditions, where additional 27.27% of residual oil (Sor) was recovered. This confirmed the potential of SPLs for applications in microbial enhanced oil recovery.

KW - biosurfactant

KW - sophorolipids

KW - Candida bombicola

KW - microbial enhanced oil recovery

KW - core-flood

KW - experiment

U2 - 10.3389/fmicb.2015.01324

DO - 10.3389/fmicb.2015.01324

M3 - Article

VL - 6

JO - Frontiers in Microbiology

T2 - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -