Sonodynamic inactivation of Gram-positive and Gram-negativebacteria using a Rose Bengal–antimicrobial peptide conjugate

John F Callan, David Costley, Heather Nesbitt, Nigel Ternan, James Dooley, Ying-Ying Huang, Michale R Hamblin, Anthony P McHale

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17 Citations (Scopus)

Abstract

Combating antimicrobial resistance is one of the most serious public health challenges facing society today.The development of new antibiotics or alternative techniques that can help combat antimicrobial resistance is being prioritised by many governments and stakeholders across the globe. Antimicrobial photodynamic therapy is one such technique that has received considerable attention but is limited by the inability of light to penetrate through human tissue, reducing its effectiveness when used to treat deep-seated infections. The related technique sonodynamic therapy (SDT) has the potential to overcome this limitation given the ability of low-intensity ultrasound to penetrate human tissue. In this study,a Rose Bengal–antimicrobial peptide conjugate was prepared for use in antimicrobial SDT (ASDT). When Staphylococcus aureus and Pseudomonas aeruginosa planktonic cultures were treated with the conjugate and subsequently exposed to ultrasound, 5 log and 7 log reductions, respectively, in bacterial numbers were observed. The conjugate also displayed improved uptake by bacterial cells compared with a mammalian cell line (P ≤ 0.01), whilst pre-treatment of a P. aeruginosa biofilm with ultrasound resulted in a 2.6-fold improvement in sensitiser diffusion (P ≤ 0.01). A preliminary in vivo experiment involving ASDT treatment of P. aeruginosa-infected wounds in mice demonstrated that ultrasound irradiation of conjugate treated wounds affects a substantial reduction in bacterial burden. Combined, the results obtained from this study highlight ASDT as a targeted broad-spectrum novel modality with potential for the treatment of deep-seated bacterial infections.
LanguageEnglish
Pages31-36
JournalInternational Journal of Antimicrobial Agents
Volume49
Issue number1
Early online date17 Nov 2016
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint

Rose Bengal
Peptides
Pseudomonas aeruginosa
Therapeutics
Photochemotherapy
Wounds and Injuries
Biofilms
Bacterial Infections
Staphylococcus aureus
Public Health
Anti-Bacterial Agents
Light
Cell Line
Infection

Keywords

  • Sonodynamic therapy
  • Antimicrobial
  • Sensitiser
  • Peptide

Cite this

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abstract = "Combating antimicrobial resistance is one of the most serious public health challenges facing society today.The development of new antibiotics or alternative techniques that can help combat antimicrobial resistance is being prioritised by many governments and stakeholders across the globe. Antimicrobial photodynamic therapy is one such technique that has received considerable attention but is limited by the inability of light to penetrate through human tissue, reducing its effectiveness when used to treat deep-seated infections. The related technique sonodynamic therapy (SDT) has the potential to overcome this limitation given the ability of low-intensity ultrasound to penetrate human tissue. In this study,a Rose Bengal–antimicrobial peptide conjugate was prepared for use in antimicrobial SDT (ASDT). When Staphylococcus aureus and Pseudomonas aeruginosa planktonic cultures were treated with the conjugate and subsequently exposed to ultrasound, 5 log and 7 log reductions, respectively, in bacterial numbers were observed. The conjugate also displayed improved uptake by bacterial cells compared with a mammalian cell line (P ≤ 0.01), whilst pre-treatment of a P. aeruginosa biofilm with ultrasound resulted in a 2.6-fold improvement in sensitiser diffusion (P ≤ 0.01). A preliminary in vivo experiment involving ASDT treatment of P. aeruginosa-infected wounds in mice demonstrated that ultrasound irradiation of conjugate treated wounds affects a substantial reduction in bacterial burden. Combined, the results obtained from this study highlight ASDT as a targeted broad-spectrum novel modality with potential for the treatment of deep-seated bacterial infections.",
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AU - Costley, David

AU - Nesbitt, Heather

AU - Ternan, Nigel

AU - Dooley, James

AU - Huang, Ying-Ying

AU - Hamblin, Michale R

AU - McHale, Anthony P

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