Soluble P-selectin levels during normal pregnancy: a longitudinal study

VA Holmes, Julie Wallace, WS Gilmore, P McFaul, HD Alexander

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Objective To investigate soluble P-selectin (sP-selectin) levels and platelet parameters in normal pregnant women compared with non-pregnant control subjects. Design A longitudinal case-control study. Setting Obstetric outpatient clinic in the Jubilee Maternity Hospital, Belfast. Population One hundred and twenty normal pregnant women and 41 non-pregnant age-matched control subjects. Methods The plasma concentration of sP-selectin in pregnant women sampled at 12, 20 and 35 weeks of gestation, and, in a subgroup at three days postpartum, and non-pregnant controls sampled in parallel, was determined using a commercial quantitative sandwich immunoassay kit. Platelet parameters on each blood sample were also recorded using a SYSMEX SE 9500 analyser. Main outcome measures Plasma sP-selectin as a measure of platelet activation in normal pregnancy. Results Soluble P-selectin was significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation, (P < 0.01, and P < 0.001, respectively). Correlation analyses showed positive correlation between sP-selectin and platelet count in pregnant women at 20 and 35 weeks of gestation (r = 0.247, P < 0.05 and r = 0.360, P < 0.001, respectively). Soluble P-selectin concentration per platelet was also significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation (P < 0.001). Conclusions Our results show that sP-selectin concentration is significantly higher in the second and third trimester of pregnancy when compared with non-pregnant control subjects sampled in parallel. This finding clarifies previous conflicting results on platelet activation in normal pregnancy, and is in agreement with those earlier studies which reported, using other methods, increased platelet activation in normal pregnancy.
    LanguageEnglish
    Pages997-1002
    JournalBjog-an International Journal of Obstetrics and Gynaecology
    Volume109
    Issue number9
    Publication statusPublished - Sep 2002

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    P-Selectin
    Longitudinal Studies
    Pregnant Women
    Pregnancy
    Platelet Activation
    Blood Platelets
    Maternity Hospitals
    Third Pregnancy Trimester
    Second Pregnancy Trimester
    Ambulatory Care Facilities
    Platelet Count
    Immunoassay
    Postpartum Period
    Obstetrics
    Case-Control Studies
    Outcome Assessment (Health Care)

    Cite this

    Holmes, VA., Wallace, J., Gilmore, WS., McFaul, P., & Alexander, HD. (2002). Soluble P-selectin levels during normal pregnancy: a longitudinal study. Bjog-an International Journal of Obstetrics and Gynaecology, 109(9), 997-1002.
    Holmes, VA ; Wallace, Julie ; Gilmore, WS ; McFaul, P ; Alexander, HD. / Soluble P-selectin levels during normal pregnancy: a longitudinal study. In: Bjog-an International Journal of Obstetrics and Gynaecology. 2002 ; Vol. 109, No. 9. pp. 997-1002.
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    abstract = "Objective To investigate soluble P-selectin (sP-selectin) levels and platelet parameters in normal pregnant women compared with non-pregnant control subjects. Design A longitudinal case-control study. Setting Obstetric outpatient clinic in the Jubilee Maternity Hospital, Belfast. Population One hundred and twenty normal pregnant women and 41 non-pregnant age-matched control subjects. Methods The plasma concentration of sP-selectin in pregnant women sampled at 12, 20 and 35 weeks of gestation, and, in a subgroup at three days postpartum, and non-pregnant controls sampled in parallel, was determined using a commercial quantitative sandwich immunoassay kit. Platelet parameters on each blood sample were also recorded using a SYSMEX SE 9500 analyser. Main outcome measures Plasma sP-selectin as a measure of platelet activation in normal pregnancy. Results Soluble P-selectin was significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation, (P < 0.01, and P < 0.001, respectively). Correlation analyses showed positive correlation between sP-selectin and platelet count in pregnant women at 20 and 35 weeks of gestation (r = 0.247, P < 0.05 and r = 0.360, P < 0.001, respectively). Soluble P-selectin concentration per platelet was also significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation (P < 0.001). Conclusions Our results show that sP-selectin concentration is significantly higher in the second and third trimester of pregnancy when compared with non-pregnant control subjects sampled in parallel. This finding clarifies previous conflicting results on platelet activation in normal pregnancy, and is in agreement with those earlier studies which reported, using other methods, increased platelet activation in normal pregnancy.",
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    Holmes, VA, Wallace, J, Gilmore, WS, McFaul, P & Alexander, HD 2002, 'Soluble P-selectin levels during normal pregnancy: a longitudinal study', Bjog-an International Journal of Obstetrics and Gynaecology, vol. 109, no. 9, pp. 997-1002.

    Soluble P-selectin levels during normal pregnancy: a longitudinal study. / Holmes, VA; Wallace, Julie; Gilmore, WS; McFaul, P; Alexander, HD.

    In: Bjog-an International Journal of Obstetrics and Gynaecology, Vol. 109, No. 9, 09.2002, p. 997-1002.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Soluble P-selectin levels during normal pregnancy: a longitudinal study

    AU - Holmes, VA

    AU - Wallace, Julie

    AU - Gilmore, WS

    AU - McFaul, P

    AU - Alexander, HD

    PY - 2002/9

    Y1 - 2002/9

    N2 - Objective To investigate soluble P-selectin (sP-selectin) levels and platelet parameters in normal pregnant women compared with non-pregnant control subjects. Design A longitudinal case-control study. Setting Obstetric outpatient clinic in the Jubilee Maternity Hospital, Belfast. Population One hundred and twenty normal pregnant women and 41 non-pregnant age-matched control subjects. Methods The plasma concentration of sP-selectin in pregnant women sampled at 12, 20 and 35 weeks of gestation, and, in a subgroup at three days postpartum, and non-pregnant controls sampled in parallel, was determined using a commercial quantitative sandwich immunoassay kit. Platelet parameters on each blood sample were also recorded using a SYSMEX SE 9500 analyser. Main outcome measures Plasma sP-selectin as a measure of platelet activation in normal pregnancy. Results Soluble P-selectin was significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation, (P < 0.01, and P < 0.001, respectively). Correlation analyses showed positive correlation between sP-selectin and platelet count in pregnant women at 20 and 35 weeks of gestation (r = 0.247, P < 0.05 and r = 0.360, P < 0.001, respectively). Soluble P-selectin concentration per platelet was also significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation (P < 0.001). Conclusions Our results show that sP-selectin concentration is significantly higher in the second and third trimester of pregnancy when compared with non-pregnant control subjects sampled in parallel. This finding clarifies previous conflicting results on platelet activation in normal pregnancy, and is in agreement with those earlier studies which reported, using other methods, increased platelet activation in normal pregnancy.

    AB - Objective To investigate soluble P-selectin (sP-selectin) levels and platelet parameters in normal pregnant women compared with non-pregnant control subjects. Design A longitudinal case-control study. Setting Obstetric outpatient clinic in the Jubilee Maternity Hospital, Belfast. Population One hundred and twenty normal pregnant women and 41 non-pregnant age-matched control subjects. Methods The plasma concentration of sP-selectin in pregnant women sampled at 12, 20 and 35 weeks of gestation, and, in a subgroup at three days postpartum, and non-pregnant controls sampled in parallel, was determined using a commercial quantitative sandwich immunoassay kit. Platelet parameters on each blood sample were also recorded using a SYSMEX SE 9500 analyser. Main outcome measures Plasma sP-selectin as a measure of platelet activation in normal pregnancy. Results Soluble P-selectin was significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation, (P < 0.01, and P < 0.001, respectively). Correlation analyses showed positive correlation between sP-selectin and platelet count in pregnant women at 20 and 35 weeks of gestation (r = 0.247, P < 0.05 and r = 0.360, P < 0.001, respectively). Soluble P-selectin concentration per platelet was also significantly higher in pregnant women than in non-pregnant control subjects at 20 and 35 weeks of gestation (P < 0.001). Conclusions Our results show that sP-selectin concentration is significantly higher in the second and third trimester of pregnancy when compared with non-pregnant control subjects sampled in parallel. This finding clarifies previous conflicting results on platelet activation in normal pregnancy, and is in agreement with those earlier studies which reported, using other methods, increased platelet activation in normal pregnancy.

    M3 - Article

    VL - 109

    SP - 997

    EP - 1002

    JO - BJOG: An International Journal of Obstetrics and Gynaecology

    T2 - BJOG: An International Journal of Obstetrics and Gynaecology

    JF - BJOG: An International Journal of Obstetrics and Gynaecology

    SN - 1470-0328

    IS - 9

    ER -

    Holmes VA, Wallace J, Gilmore WS, McFaul P, Alexander HD. Soluble P-selectin levels during normal pregnancy: a longitudinal study. Bjog-an International Journal of Obstetrics and Gynaecology. 2002 Sep;109(9):997-1002.