Solar water disinfection (SODIS): Impact on hepatitis A virus and on a human norovirus surrogate under natural solar conditions

David Polo, Irene García-Fernández, Pilar Fernández-Ibáñez, Jesús L. Romalde

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This study evaluates the effectiveness of solar water disinfection (SODIS) in the reduction and inactivation of hepatitis A virus (HAV) and of the human Norovirus surrogate, murine Norovirus (MNV-1), under natural solar conditions. Experiments were performed in 330 ml polyethylene terephthalate (PET) bottles containing HAV or MNV-1 contaminated waters (103 PFU/ml) that were exposed to natural sunlight for 2 to 8 h. Parallel experiments under controlled temperature and/ or in darkness conditions were also included. Samples were concentrated by electropositive charged filters and analysed by RT-real time PCR (RT-qPCR) and infectivity assays. Temperature reached in bottles throughout the exposure period ranged from 22 to 40° C. After 8 h of solar exposure (cumulative UV dose of ~828 kJ/m2 and UV irradiance of ~20 kJ/l), the results showed significant (P < 0.05) reductions from 4.0 (±0.56) ×l04 to 3.15 (±0.69) × 103 RNA copies/100 ml (92.1%, 1.1 log) for HAV and from 5.91 (±0.59) × 104 to 9.24 (±3.91) × 103 RNA copies/100 ml (84.4%, 0.81 log) for MNV-1. SODIS conditions induced a loss of infectivity between 33.4% and 83.4% after 4 to 8 h in HAV trials, and between 33.4% and 66.7% after 6 h to 8 h in MNV-1 trials. The results obtained indicated a greater importance of sunlight radiation over the temperature as the main factor for viral reduction.

LanguageEnglish
Pages41-49
Number of pages9
JournalInternational Microbiology
Volume18
Issue number1
DOIs
Publication statusPublished - 14 Sep 2015

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Norovirus
Hepatitis A virus
Disinfection
Sunlight
Temperature
Water
Human hepatitis A virus
RNA
Polyethylene Terephthalates
Darkness
Real-Time Polymerase Chain Reaction
Radiation

Keywords

  • Hepatitis A virus (HAV)
  • Murine Norovirus (MNV-1)
  • Solar water disinfection (SODIS)
  • Water disinfection

Cite this

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title = "Solar water disinfection (SODIS): Impact on hepatitis A virus and on a human norovirus surrogate under natural solar conditions",
abstract = "This study evaluates the effectiveness of solar water disinfection (SODIS) in the reduction and inactivation of hepatitis A virus (HAV) and of the human Norovirus surrogate, murine Norovirus (MNV-1), under natural solar conditions. Experiments were performed in 330 ml polyethylene terephthalate (PET) bottles containing HAV or MNV-1 contaminated waters (103 PFU/ml) that were exposed to natural sunlight for 2 to 8 h. Parallel experiments under controlled temperature and/ or in darkness conditions were also included. Samples were concentrated by electropositive charged filters and analysed by RT-real time PCR (RT-qPCR) and infectivity assays. Temperature reached in bottles throughout the exposure period ranged from 22 to 40° C. After 8 h of solar exposure (cumulative UV dose of ~828 kJ/m2 and UV irradiance of ~20 kJ/l), the results showed significant (P < 0.05) reductions from 4.0 (±0.56) ×l04 to 3.15 (±0.69) × 103 RNA copies/100 ml (92.1{\%}, 1.1 log) for HAV and from 5.91 (±0.59) × 104 to 9.24 (±3.91) × 103 RNA copies/100 ml (84.4{\%}, 0.81 log) for MNV-1. SODIS conditions induced a loss of infectivity between 33.4{\%} and 83.4{\%} after 4 to 8 h in HAV trials, and between 33.4{\%} and 66.7{\%} after 6 h to 8 h in MNV-1 trials. The results obtained indicated a greater importance of sunlight radiation over the temperature as the main factor for viral reduction.",
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Solar water disinfection (SODIS) : Impact on hepatitis A virus and on a human norovirus surrogate under natural solar conditions. / Polo, David; García-Fernández, Irene; Fernández-Ibáñez, Pilar; Romalde, Jesús L.

In: International Microbiology, Vol. 18, No. 1, 14.09.2015, p. 41-49.

Research output: Contribution to journalArticle

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