Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures

L Marenah, Peter Flatt, DF Orr, Stephen McClean, C Shaw, Yasser Abdel-Wahab

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p
Original languageEnglish
Pages (from-to)315-321
JournalBiological Chemistry
Volume385
Issue number3-4
Publication statusPublished - Mar 2004

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