Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures

L Marenah, Peter Flatt, DF Orr, Stephen McClean, C Shaw, Yasser Abdel-Wahab

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18 Citations (Scopus)

Abstract

Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p
LanguageEnglish
Pages315-321
JournalBiological Chemistry
Volume385
Issue number3-4
Publication statusPublished - Mar 2004

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Bombesin
Anura
Insulin
Skin
Peptides
Electric Stimulation
Young Adult
High Pressure Liquid Chromatography
Glucose

Cite this

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abstract = "Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p",
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AU - Flatt, Peter

AU - Orr, DF

AU - McClean, Stephen

AU - Shaw, C

AU - Abdel-Wahab, Yasser

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AB - Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p

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EP - 321

JO - Biological Chemistry

T2 - Biological Chemistry

JF - Biological Chemistry

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