Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures

L Marenah; Peter Flatt; DF Orr; Stephen McClean; C Shaw; Yasser Abdel-Wahab

Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures

L Marenah, Peter Flatt, DF Orr, Stephen McClean, C Shaw, Yasser Abdel-Wahab

Research output: Contribution to journal › Article › peer-review

Abstract

Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p

Original language	English
Pages (from-to)	315-321
Journal	Biological Chemistry
Volume	385
Issue number	3-4
Publication status	Published (in print/issue) - Mar 2004

Bibliographical note

Conference on Oxygen and the Cell, Berlin, GERMANY, SEP, 2003

Cite this

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title = "Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures",

abstract = "Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p",

author = "L Marenah and Peter Flatt and DF Orr and Stephen McClean and C Shaw and Yasser Abdel-Wahab",

note = "Conference on Oxygen and the Cell, Berlin, GERMANY, SEP, 2003",

year = "2004",

month = mar,

language = "English",

volume = "385",

pages = "315--321",

journal = "Biological Chemistry",

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T1 - Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures

AU - Marenah, L

AU - Flatt, Peter

AU - Orr, DF

AU - McClean, Stephen

AU - Shaw, C

AU - Abdel-Wahab, Yasser

N1 - Conference on Oxygen and the Cell, Berlin, GERMANY, SEP, 2003

PY - 2004/3

Y1 - 2004/3

N2 - Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p

AB - Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p

M3 - Article

SN - 1437-4315

VL - 385

SP - 315

EP - 321

JO - Biological Chemistry

JF - Biological Chemistry

IS - 3-4

ER -

Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures

Abstract

Bibliographical note

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