Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p
|Publication status||Published - Mar 2004|
Marenah, L., Flatt, P., Orr, DF., McClean, S., Shaw, C., & Abdel-Wahab, Y. (2004). Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures. Biological Chemistry, 385(3-4), 315-321.