Single‐Step Primary Amine Synthesis on Proton Sensitive Nanofilms to Overcome Its Debye Length Limitations

Chia‐Ming Yang, Hui‐Ling Liu, Chih‐Ching Ho, Hsieh‐Fu Tsai, Nikhil Bhalla

Research output: Contribution to journalArticlepeer-review

15 Downloads (Pure)


The debye length is a measure of the distance over which the electric field of a charged particle decays in an electrolyte solution. If the binding of the analyte to the surface of the transducer is too far away from the surface, the electric field to the analyte may decay over a distance greater than the debye length thereby reducing the sensitivity of the measurement. In this context, this study has developed a simple one‐step protein immobilization strategy to covalently attach proteins on the sensor surface. Our binding strategy, which uses hydrogen peroxide (H2O2) ensures that the analyte is attached as close as possible to the transducer surface. This study evaluates our findings by comparing our strategy with silane chemistry and elucidating the debye length effects with colorimetric assays and field effect devices. Additionally, as a case study, we also evaluated the performance of our methodology for the detection of glucose oxidation by a field effect device. Overall, the developed immobilization strategy avoids the effects of the debye length and improves the performance of the biosensor.
Original languageEnglish
Article number2300080
Pages (from-to)1-9
Number of pages9
JournalAdvanced Materials Interfaces
Issue number21
Early online date30 Jun 2023
Publication statusPublished (in print/issue) - 26 Jul 2023

Bibliographical note

Funding Information:
The authors would like to thank support from Royal Society, grant number IEC\R3\193004, International Exchanges Cost Sharing Programme.

Publisher Copyright:
© 2023 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.


  • amines
  • field‐effect transistor
  • debye‐length
  • proteins
  • biosensors
  • debye-length
  • field-effect transistor


Dive into the research topics of 'Single‐Step Primary Amine Synthesis on Proton Sensitive Nanofilms to Overcome Its Debye Length Limitations'. Together they form a unique fingerprint.

Cite this