Simultaneous Saccharification and Fermentation of Kanlow Switchgrass Pretreated by Hydrothermolysis Using Kluyveromyces marxianus IMB4

Lilis Suryawati, Mark R. Wilkins, Danielle D. Bellmer, Raymond L. Huhnke, Niels O. Maness, Ibrahim Banat

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A thermotolerant yeast strain named Kluyveromyces marxianus IMB4 was used in a simultaneous saccharification and fermentation (SSF) process using Kanlow switchgrass as a feedstock. Switchgrass was pretreated using hydrothermolysis at 200 degrees C for 10 min. After pretreatment, insoluble solids were separated from the liquid prehydrolyzate by filtration and washed with deionized water to remove soluble sugars and inhibitors. Insoluble solids were then hydrolyzed using a commercial cellulase preparation and the released glucose was fermented to ethanol by K. marxianus IMB4 in an SSF process. SSF temperature was 37, 41, or 45 degrees C and PH was 4.8 or 5.5. SSF was conducted for 7 days. Results were compared with a control of Saccharomyces cerevisiae D(5)A at 37 degrees C and PH 4.8. Fermentation by IMB4 at 45 and 41 degrees C ceased after 3 and 4 days, respectively, when a PH 4.8 citrate buffer was used. Fermentation continued for all 7 days using IMB4 at 37 degrees C and the control. When pH 5.5 citrate buffer was used, fermentation ceased after 96 h using IMB4 at 45 degrees C, and ethanol yield was greater than when PH 4.8 citrate buffer was used (78% theoretical). Ethanol yield using IMB4 at 45 degrees C, PH 5.5 was greater than the control after 48, 72, and 96 h (P < 0.05).
LanguageEnglish
Pages894-902
JournalBiotechnology and Bioengineering
Volume101
Issue number5
DOIs
Publication statusPublished - Dec 2008

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Kluyveromyces marxianus
saccharification
Panicum virgatum
fermentation
citrates
buffers
ethanol
feedstocks
endo-1,4-beta-glucanase
Saccharomyces cerevisiae
pretreatment
yeasts
sugars
glucose
liquids

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Suryawati, Lilis ; Wilkins, Mark R. ; Bellmer, Danielle D. ; Huhnke, Raymond L. ; Maness, Niels O. ; Banat, Ibrahim. / Simultaneous Saccharification and Fermentation of Kanlow Switchgrass Pretreated by Hydrothermolysis Using Kluyveromyces marxianus IMB4. In: Biotechnology and Bioengineering. 2008 ; Vol. 101, No. 5. pp. 894-902.
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abstract = "A thermotolerant yeast strain named Kluyveromyces marxianus IMB4 was used in a simultaneous saccharification and fermentation (SSF) process using Kanlow switchgrass as a feedstock. Switchgrass was pretreated using hydrothermolysis at 200 degrees C for 10 min. After pretreatment, insoluble solids were separated from the liquid prehydrolyzate by filtration and washed with deionized water to remove soluble sugars and inhibitors. Insoluble solids were then hydrolyzed using a commercial cellulase preparation and the released glucose was fermented to ethanol by K. marxianus IMB4 in an SSF process. SSF temperature was 37, 41, or 45 degrees C and PH was 4.8 or 5.5. SSF was conducted for 7 days. Results were compared with a control of Saccharomyces cerevisiae D(5)A at 37 degrees C and PH 4.8. Fermentation by IMB4 at 45 and 41 degrees C ceased after 3 and 4 days, respectively, when a PH 4.8 citrate buffer was used. Fermentation continued for all 7 days using IMB4 at 37 degrees C and the control. When pH 5.5 citrate buffer was used, fermentation ceased after 96 h using IMB4 at 45 degrees C, and ethanol yield was greater than when PH 4.8 citrate buffer was used (78{\%} theoretical). Ethanol yield using IMB4 at 45 degrees C, PH 5.5 was greater than the control after 48, 72, and 96 h (P < 0.05).",
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Simultaneous Saccharification and Fermentation of Kanlow Switchgrass Pretreated by Hydrothermolysis Using Kluyveromyces marxianus IMB4. / Suryawati, Lilis; Wilkins, Mark R.; Bellmer, Danielle D.; Huhnke, Raymond L.; Maness, Niels O.; Banat, Ibrahim.

In: Biotechnology and Bioengineering, Vol. 101, No. 5, 12.2008, p. 894-902.

Research output: Contribution to journalArticle

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