Simultaneous saccharification and fermentation of Kanlow switchgrass by thermotolerant Kluyveromyces marxianus IMB3: The effect of enzyme loading, temperature and higher solid loadings

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Abstract

Switchgrass (Panicum virgatum) was subjected to hydrothermolysis pretreatment and then used to study the effect of enzyme loading and temperature in a simultaneous saccharification and fermentation (SSF) with the thermotolerant yeast strain Kluyveromyces marxianus IMB3 at 8% solid loading. Various loadings of Accellerase 1500 between 0.1 and 1.1 mL g-1 glucan were tested in SSF at 45 C (activity of enzyme was 82.2 FPU mL-1). The optimum enzyme loading was 0.7 mL g-1 glucan based on the six different enzyme loadings tested. SSFs were performed at 37, 41 and 45 C with an enzyme loading of 0.7 mL g-1 glucan. The highest ethanol concentration of 22.5 g L-1 was obtained after 168 h with SSF at 45C, which was equivalent to 86% yield. Four different batch and fed-batch strategies were evaluated using a total solid loading of 12% (dry basis). About 32 g L-1 ethanol was produced with the four strategies, which was equivalent to 82% yield.
LanguageEnglish
Pages10618-10624
JournalBioresource Technology
Volume102
DOIs
Publication statusPublished - 15 Sep 2011

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Kluyveromyces marxianus
saccharification
Panicum virgatum
glucans
fermentation
enzymes
temperature
ethanol
total solids
pretreatment
enzyme activity
yeasts

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title = "Simultaneous saccharification and fermentation of Kanlow switchgrass by thermotolerant Kluyveromyces marxianus IMB3: The effect of enzyme loading, temperature and higher solid loadings",
abstract = "Switchgrass (Panicum virgatum) was subjected to hydrothermolysis pretreatment and then used to study the effect of enzyme loading and temperature in a simultaneous saccharification and fermentation (SSF) with the thermotolerant yeast strain Kluyveromyces marxianus IMB3 at 8{\%} solid loading. Various loadings of Accellerase 1500 between 0.1 and 1.1 mL g-1 glucan were tested in SSF at 45 C (activity of enzyme was 82.2 FPU mL-1). The optimum enzyme loading was 0.7 mL g-1 glucan based on the six different enzyme loadings tested. SSFs were performed at 37, 41 and 45 C with an enzyme loading of 0.7 mL g-1 glucan. The highest ethanol concentration of 22.5 g L-1 was obtained after 168 h with SSF at 45C, which was equivalent to 86{\%} yield. Four different batch and fed-batch strategies were evaluated using a total solid loading of 12{\%} (dry basis). About 32 g L-1 ethanol was produced with the four strategies, which was equivalent to 82{\%} yield.",
author = "Ibrahim Banat",
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T1 - Simultaneous saccharification and fermentation of Kanlow switchgrass by thermotolerant Kluyveromyces marxianus IMB3: The effect of enzyme loading, temperature and higher solid loadings

AU - Banat, Ibrahim

PY - 2011/9/15

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N2 - Switchgrass (Panicum virgatum) was subjected to hydrothermolysis pretreatment and then used to study the effect of enzyme loading and temperature in a simultaneous saccharification and fermentation (SSF) with the thermotolerant yeast strain Kluyveromyces marxianus IMB3 at 8% solid loading. Various loadings of Accellerase 1500 between 0.1 and 1.1 mL g-1 glucan were tested in SSF at 45 C (activity of enzyme was 82.2 FPU mL-1). The optimum enzyme loading was 0.7 mL g-1 glucan based on the six different enzyme loadings tested. SSFs were performed at 37, 41 and 45 C with an enzyme loading of 0.7 mL g-1 glucan. The highest ethanol concentration of 22.5 g L-1 was obtained after 168 h with SSF at 45C, which was equivalent to 86% yield. Four different batch and fed-batch strategies were evaluated using a total solid loading of 12% (dry basis). About 32 g L-1 ethanol was produced with the four strategies, which was equivalent to 82% yield.

AB - Switchgrass (Panicum virgatum) was subjected to hydrothermolysis pretreatment and then used to study the effect of enzyme loading and temperature in a simultaneous saccharification and fermentation (SSF) with the thermotolerant yeast strain Kluyveromyces marxianus IMB3 at 8% solid loading. Various loadings of Accellerase 1500 between 0.1 and 1.1 mL g-1 glucan were tested in SSF at 45 C (activity of enzyme was 82.2 FPU mL-1). The optimum enzyme loading was 0.7 mL g-1 glucan based on the six different enzyme loadings tested. SSFs were performed at 37, 41 and 45 C with an enzyme loading of 0.7 mL g-1 glucan. The highest ethanol concentration of 22.5 g L-1 was obtained after 168 h with SSF at 45C, which was equivalent to 86% yield. Four different batch and fed-batch strategies were evaluated using a total solid loading of 12% (dry basis). About 32 g L-1 ethanol was produced with the four strategies, which was equivalent to 82% yield.

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