SIMULTANEOUS RAW STARCH HYDROLYSIS AND ETHANOL FERMENTATION BY GLUCOAMYLASE FROM RHIZOCTONIA-SOLANI AND SACCHAROMYCES-CEREVISIAE

Crude glucoamylase preparation from Rhizoctonia solani was used to saccharify raw and cooked starch. Various concentrations of potato starch and wheat flour from 10-40%, w/v were used for mashing but 30% was found to be the optimal and economical. The saccharified mash yielded 5.89%, v/v ethanol in a simultaneous saccharification and fermentation process using a yeast strain Saccharomyces cerevisae (SC-39) at 35 degrees C for 4 days. Removal of inhibitory substances from the fermenting broth through dialysis caused considerable increase in ethanol production.