Simultaneous determination of estrogenic and androgenic hormones in water by isotope dilution gas chromatography–tandem mass spectrometry

Trang Trinh, Nick B. Harden, Heather Coleman, Stuart J. Khan

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A rapid gas chromatography–tandem mass spectrometry (GC–MS/MS) analytical method was developedfor the simultaneous analysis of 7 estrogenic hormones (17-estradiol, 17-estradiol, estrone, mestranol,17-ethynylestradiol, levonorgestrel, estriol) and 5 androgenic hormones (testosterone, androsterone,etiocholanolone, dihydrotestosterone, androstenedione) in aqueous matrices. This method is unique inits inclusion of all 12 of these estrogens and androgens and is of particular value due to its very shortchromatographic run time of 15 min. The use of isotope dilution for all analytes ensures the accurate quantification, accounting for analytical variabilities that may be introduced during sample processing andinstrumental analysis. Direct isotopically labelled analogues were used for 8 of the 12 hormones and satisfactory isotope standards were identified for the remaining 4 hormones. Method detection levels (MDLs)were determined to describe analyte concentrations sufficient to provide a signal with 99% certaintyof detection. The established MDLs for most analytes were 1–5 ng L−1 in a variety of aqueous matrices.However, slightly higher MDLs were observed for etiocholanolone, androstenedione, testosterone, levonorgestrel and dihydrotestosterone in some aqueous matrices. Sample matrices were observed to haveonly a minor impact on MDLs and the method validation confirmed satisfactory method stability overintra-day and inter-day analyses of surface water and tertiary treated effluent samples.
LanguageEnglish
Pages1668-1676
JournalJOURNAL OF CHROMATOGRAPHY A
Volume1218
Issue number12
Early online date31 Jan 2011
DOIs
Publication statusE-pub ahead of print - 31 Jan 2011

Fingerprint

Etiocholanolone
Tandem Mass Spectrometry
Isotopes
Gas chromatography
Gas Chromatography-Mass Spectrometry
Dilution
Mass spectrometry
Dihydrotestosterone
Hormones
Androsterone
Levonorgestrel
Water
Testosterone
Estradiol
Mestranol
Estriol
Estrone
Surface waters
Androgens
Effluents

Keywords

  • Estrogens
  • Androgens
  • Drinking water
  • Wastewater
  • Surface water

Cite this

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abstract = "A rapid gas chromatography–tandem mass spectrometry (GC–MS/MS) analytical method was developedfor the simultaneous analysis of 7 estrogenic hormones (17-estradiol, 17-estradiol, estrone, mestranol,17-ethynylestradiol, levonorgestrel, estriol) and 5 androgenic hormones (testosterone, androsterone,etiocholanolone, dihydrotestosterone, androstenedione) in aqueous matrices. This method is unique inits inclusion of all 12 of these estrogens and androgens and is of particular value due to its very shortchromatographic run time of 15 min. The use of isotope dilution for all analytes ensures the accurate quantification, accounting for analytical variabilities that may be introduced during sample processing andinstrumental analysis. Direct isotopically labelled analogues were used for 8 of the 12 hormones and satisfactory isotope standards were identified for the remaining 4 hormones. Method detection levels (MDLs)were determined to describe analyte concentrations sufficient to provide a signal with 99{\%} certaintyof detection. The established MDLs for most analytes were 1–5 ng L−1 in a variety of aqueous matrices.However, slightly higher MDLs were observed for etiocholanolone, androstenedione, testosterone, levonorgestrel and dihydrotestosterone in some aqueous matrices. Sample matrices were observed to haveonly a minor impact on MDLs and the method validation confirmed satisfactory method stability overintra-day and inter-day analyses of surface water and tertiary treated effluent samples.",
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Simultaneous determination of estrogenic and androgenic hormones in water by isotope dilution gas chromatography–tandem mass spectrometry. / Trinh, Trang; Harden, Nick B.; Coleman, Heather; Khan, Stuart J.

In: JOURNAL OF CHROMATOGRAPHY A, Vol. 1218, No. 12, 31.01.2011, p. 1668-1676.

Research output: Contribution to journalArticle

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T1 - Simultaneous determination of estrogenic and androgenic hormones in water by isotope dilution gas chromatography–tandem mass spectrometry

AU - Trinh, Trang

AU - Harden, Nick B.

AU - Coleman, Heather

AU - Khan, Stuart J.

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N2 - A rapid gas chromatography–tandem mass spectrometry (GC–MS/MS) analytical method was developedfor the simultaneous analysis of 7 estrogenic hormones (17-estradiol, 17-estradiol, estrone, mestranol,17-ethynylestradiol, levonorgestrel, estriol) and 5 androgenic hormones (testosterone, androsterone,etiocholanolone, dihydrotestosterone, androstenedione) in aqueous matrices. This method is unique inits inclusion of all 12 of these estrogens and androgens and is of particular value due to its very shortchromatographic run time of 15 min. The use of isotope dilution for all analytes ensures the accurate quantification, accounting for analytical variabilities that may be introduced during sample processing andinstrumental analysis. Direct isotopically labelled analogues were used for 8 of the 12 hormones and satisfactory isotope standards were identified for the remaining 4 hormones. Method detection levels (MDLs)were determined to describe analyte concentrations sufficient to provide a signal with 99% certaintyof detection. The established MDLs for most analytes were 1–5 ng L−1 in a variety of aqueous matrices.However, slightly higher MDLs were observed for etiocholanolone, androstenedione, testosterone, levonorgestrel and dihydrotestosterone in some aqueous matrices. Sample matrices were observed to haveonly a minor impact on MDLs and the method validation confirmed satisfactory method stability overintra-day and inter-day analyses of surface water and tertiary treated effluent samples.

AB - A rapid gas chromatography–tandem mass spectrometry (GC–MS/MS) analytical method was developedfor the simultaneous analysis of 7 estrogenic hormones (17-estradiol, 17-estradiol, estrone, mestranol,17-ethynylestradiol, levonorgestrel, estriol) and 5 androgenic hormones (testosterone, androsterone,etiocholanolone, dihydrotestosterone, androstenedione) in aqueous matrices. This method is unique inits inclusion of all 12 of these estrogens and androgens and is of particular value due to its very shortchromatographic run time of 15 min. The use of isotope dilution for all analytes ensures the accurate quantification, accounting for analytical variabilities that may be introduced during sample processing andinstrumental analysis. Direct isotopically labelled analogues were used for 8 of the 12 hormones and satisfactory isotope standards were identified for the remaining 4 hormones. Method detection levels (MDLs)were determined to describe analyte concentrations sufficient to provide a signal with 99% certaintyof detection. The established MDLs for most analytes were 1–5 ng L−1 in a variety of aqueous matrices.However, slightly higher MDLs were observed for etiocholanolone, androstenedione, testosterone, levonorgestrel and dihydrotestosterone in some aqueous matrices. Sample matrices were observed to haveonly a minor impact on MDLs and the method validation confirmed satisfactory method stability overintra-day and inter-day analyses of surface water and tertiary treated effluent samples.

KW - Estrogens

KW - Androgens

KW - Drinking water

KW - Wastewater

KW - Surface water

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SN - 0021-9673

IS - 12

ER -