Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes

Shauna M. Keane; Eamonn P. Culligan; Roland F. Hoffmann; Cormac G. M. Gahan; Colin Hill; William J. Snelling; Roy D. Sleator

doi:10.1080/21655979.2016.1171438

Shedding light on betL: pPL2-lux mediated real-time analysis of betL expression in Listeria monocytogenes

Shauna M. Keane
, Eamonn P. Culligan
, Roland F. Hoffmann
, Cormac G. M. Gahan
, Colin Hill
, William J. Snelling
, Roy D. Sleator

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σA promoter's −10 and −35 spacer results in a ‘twist’-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.

Original language	English
Pages (from-to)	116-119
Journal	Bioengineered
Volume	7
Issue number	2
Early online date	20 May 2016
DOIs	https://doi.org/10.1080/21655979.2016.1171438
Publication status	Published online - 20 May 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

betL*
lux
Listeria
osmotolerance

Access to Document

10.1080/21655979.2016.1171438

https://doi.org/10.1080/21655979.2016.1171438

William Snelling
- School of Biomedical Sciences - Online Tutor (Global Online)
- Faculty Of Life & Health Sciences
- Biomedical Sciences Research
Person: Academic

Cite this

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title = "Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes",

abstract = "We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σA promoter's −10 and −35 spacer results in a {\textquoteleft}twist{\textquoteright}-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.",

keywords = "betL*, lux, Listeria, osmotolerance",

author = "Keane, \{Shauna M.\} and Culligan, \{Eamonn P.\} and Hoffmann, \{Roland F.\} and Gahan, \{Cormac G. M.\} and Colin Hill and Snelling, \{William J.\} and Sleator, \{Roy D.\}",

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AU - Keane, Shauna M.

AU - Culligan, Eamonn P.

AU - Hoffmann, Roland F.

AU - Gahan, Cormac G. M.

AU - Hill, Colin

AU - Snelling, William J.

AU - Sleator, Roy D.

PY - 2016/5/20

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AB - We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σA promoter's −10 and −35 spacer results in a ‘twist’-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.

KW - betL

KW - lux

KW - Listeria

KW - osmotolerance

U2 - 10.1080/21655979.2016.1171438

DO - 10.1080/21655979.2016.1171438

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