Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes

Shauna M. Keane, Eamonn P. Culligan, Roland F. Hoffmann, Cormac G. M. Gahan, Colin Hill, William J. Snelling, Roy D. Sleator

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    Abstract

    We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σA promoter's −10 and −35 spacer results in a ‘twist’-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.
    Original languageEnglish
    Pages (from-to)116-119
    JournalBioengineered
    Volume7
    Issue number2
    Early online date20 May 2016
    DOIs
    Publication statusE-pub ahead of print - 20 May 2016

    Keywords

    • betL*
    • lux
    • Listeria
    • osmotolerance

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    William Snelling

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