Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes

Shauna M. Keane, Eamonn P. Culligan, Roland F. Hoffmann, Cormac G. M. Gahan, Colin Hill, William J. Snelling, Roy D. Sleator

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σA promoter's −10 and −35 spacer results in a ‘twist’-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.
    LanguageEnglish
    Pages116-119
    JournalBioengineered
    Volume7
    Issue number2
    Early online date20 May 2016
    DOIs
    Publication statusE-pub ahead of print - 20 May 2016

    Fingerprint

    Thymine
    Listeria monocytogenes
    Transcriptional Activation
    Phenotype
    Mutation
    DNA

    Keywords

    • betL*
    • lux
    • Listeria
    • osmotolerance

    Cite this

    Keane, Shauna M. ; Culligan, Eamonn P. ; Hoffmann, Roland F. ; Gahan, Cormac G. M. ; Hill, Colin ; Snelling, William J. ; Sleator, Roy D. / Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes. In: Bioengineered. 2016 ; Vol. 7, No. 2. pp. 116-119.
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    Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes. / Keane, Shauna M.; Culligan, Eamonn P.; Hoffmann, Roland F.; Gahan, Cormac G. M.; Hill, Colin; Snelling, William J.; Sleator, Roy D.

    In: Bioengineered, Vol. 7, No. 2, 20.05.2016, p. 116-119.

    Research output: Contribution to journalArticle

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    AU - Keane, Shauna M.

    AU - Culligan, Eamonn P.

    AU - Hoffmann, Roland F.

    AU - Gahan, Cormac G. M.

    AU - Hill, Colin

    AU - Snelling, William J.

    AU - Sleator, Roy D.

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    KW - betL

    KW - lux

    KW - Listeria

    KW - osmotolerance

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