SEX-DIFFERENCES IN THE DIABETES-INDUCED MODULATION OF RAT HEPATIC CYTOCHROME-P450 PROTEINS

CR Barnett, S Rudd, Peter Flatt, C Ioannides

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Sex differences in the diabetes-induced changes in hepatic cytochrome P450 proteins were investigated in rats treated with streptozotocin. Changes in specific cytochrome P450 proteins were monitored using diagnostic substrates and immunologically utilizing specific polyclonal antibodies. When expressed in terms of nmoles of total cytochrome P450, ethoxyresorufin O-deethylase activity was increased by treatment with streptozotocin, the extent of induction being the same in the two sexes. In contrast, lauric acid hydroxylase and ethylmorphine N-demethylase activities were induced only in the male rat. Finally, p-nitrophenol hydroxlylase and pentoxyresorufin O-alkylase were enhanced by the same treatment in both sexes, the effect being more pronounced in the male. These findings indicate that sex-specific changes in certain cytochrome P450 proteins exist in response to insulin-dependent diabetes but these cannot, however, be ascribed to sex differences in the severity of diabetes induced by streptozotocin since the degrees of hyperketonaemia and hyperglycaemia were the same in the two sexes. These are likely to reflect sex-specific differences in growth hormone and triglyceride levels in the diabetic animals.
LanguageEnglish
Pages313-319
JournalBIiochemical Pharmacology
Volume45
Issue number2
Publication statusPublished - Jan 1993

Fingerprint

Sex Characteristics
Cytochrome P-450 Enzyme System
Liver
Streptozocin
Ethylmorphine-N-Demethylase
Proteins
Cytochrome P-450 CYP4A
Cytochrome P-450 CYP1A1
Experimental Diabetes Mellitus
Hyperglycemia
Growth Hormone
Triglycerides
Insulin
Antibodies

Cite this

@article{f520633beae9428bb552cf8286e01aac,
title = "SEX-DIFFERENCES IN THE DIABETES-INDUCED MODULATION OF RAT HEPATIC CYTOCHROME-P450 PROTEINS",
abstract = "Sex differences in the diabetes-induced changes in hepatic cytochrome P450 proteins were investigated in rats treated with streptozotocin. Changes in specific cytochrome P450 proteins were monitored using diagnostic substrates and immunologically utilizing specific polyclonal antibodies. When expressed in terms of nmoles of total cytochrome P450, ethoxyresorufin O-deethylase activity was increased by treatment with streptozotocin, the extent of induction being the same in the two sexes. In contrast, lauric acid hydroxylase and ethylmorphine N-demethylase activities were induced only in the male rat. Finally, p-nitrophenol hydroxlylase and pentoxyresorufin O-alkylase were enhanced by the same treatment in both sexes, the effect being more pronounced in the male. These findings indicate that sex-specific changes in certain cytochrome P450 proteins exist in response to insulin-dependent diabetes but these cannot, however, be ascribed to sex differences in the severity of diabetes induced by streptozotocin since the degrees of hyperketonaemia and hyperglycaemia were the same in the two sexes. These are likely to reflect sex-specific differences in growth hormone and triglyceride levels in the diabetic animals.",
author = "CR Barnett and S Rudd and Peter Flatt and C Ioannides",
year = "1993",
month = "1",
language = "English",
volume = "45",
pages = "313--319",
journal = "BIiochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier",
number = "2",

}

SEX-DIFFERENCES IN THE DIABETES-INDUCED MODULATION OF RAT HEPATIC CYTOCHROME-P450 PROTEINS. / Barnett, CR; Rudd, S; Flatt, Peter; Ioannides, C.

In: BIiochemical Pharmacology, Vol. 45, No. 2, 01.1993, p. 313-319.

Research output: Contribution to journalArticle

TY - JOUR

T1 - SEX-DIFFERENCES IN THE DIABETES-INDUCED MODULATION OF RAT HEPATIC CYTOCHROME-P450 PROTEINS

AU - Barnett, CR

AU - Rudd, S

AU - Flatt, Peter

AU - Ioannides, C

PY - 1993/1

Y1 - 1993/1

N2 - Sex differences in the diabetes-induced changes in hepatic cytochrome P450 proteins were investigated in rats treated with streptozotocin. Changes in specific cytochrome P450 proteins were monitored using diagnostic substrates and immunologically utilizing specific polyclonal antibodies. When expressed in terms of nmoles of total cytochrome P450, ethoxyresorufin O-deethylase activity was increased by treatment with streptozotocin, the extent of induction being the same in the two sexes. In contrast, lauric acid hydroxylase and ethylmorphine N-demethylase activities were induced only in the male rat. Finally, p-nitrophenol hydroxlylase and pentoxyresorufin O-alkylase were enhanced by the same treatment in both sexes, the effect being more pronounced in the male. These findings indicate that sex-specific changes in certain cytochrome P450 proteins exist in response to insulin-dependent diabetes but these cannot, however, be ascribed to sex differences in the severity of diabetes induced by streptozotocin since the degrees of hyperketonaemia and hyperglycaemia were the same in the two sexes. These are likely to reflect sex-specific differences in growth hormone and triglyceride levels in the diabetic animals.

AB - Sex differences in the diabetes-induced changes in hepatic cytochrome P450 proteins were investigated in rats treated with streptozotocin. Changes in specific cytochrome P450 proteins were monitored using diagnostic substrates and immunologically utilizing specific polyclonal antibodies. When expressed in terms of nmoles of total cytochrome P450, ethoxyresorufin O-deethylase activity was increased by treatment with streptozotocin, the extent of induction being the same in the two sexes. In contrast, lauric acid hydroxylase and ethylmorphine N-demethylase activities were induced only in the male rat. Finally, p-nitrophenol hydroxlylase and pentoxyresorufin O-alkylase were enhanced by the same treatment in both sexes, the effect being more pronounced in the male. These findings indicate that sex-specific changes in certain cytochrome P450 proteins exist in response to insulin-dependent diabetes but these cannot, however, be ascribed to sex differences in the severity of diabetes induced by streptozotocin since the degrees of hyperketonaemia and hyperglycaemia were the same in the two sexes. These are likely to reflect sex-specific differences in growth hormone and triglyceride levels in the diabetic animals.

M3 - Article

VL - 45

SP - 313

EP - 319

JO - BIiochemical Pharmacology

T2 - BIiochemical Pharmacology

JF - BIiochemical Pharmacology

SN - 0006-2952

IS - 2

ER -