Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses

A Monaghan, B Byrne, S Fanning, T Sweeny, D.A. McDowell, D J Bolton

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    Four hundred and fifty beef animal hides and a similar number of carcasses were screened for STEC in 3 beef abattoirs over a 12 month period using PCR and culture based methods. 67% (301/450) of hides and 27% (122/450) of carcasses were STEC PCR positive. Forty isolates representing 12 STEC serotypes (O5:H-,O13:H2, O26:H11, O33:H11, O55:H11, O113:H4, O128:H8, O136:H12, O138:H48, O150:H2, O168:H8 and ONT:H11) and 15 serotype-virulotype combinations were identified.This study provides much needed non-O157 STEC surveillance data and also provides further evidence of bovines as a source of clinically significant STEC as well as identifying 3 emerging serotypes O5:H- (eae-b1), O13:H2 (eae-z), and O150:H2 (eae-z) that should be considered when developing beef testing procedures for non-O157 STEC.
    Original languageEnglish
    Pages (from-to)223-229
    JournalFood Microbiology
    Issue number2
    Publication statusPublished - Dec 2012


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