Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses

A Monaghan, B Byrne, S Fanning, T Sweeny, D.A. McDowell, D J Bolton

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    Abstract

    Four hundred and fifty beef animal hides and a similar number of carcasses were screened for STEC in 3 beef abattoirs over a 12 month period using PCR and culture based methods. 67% (301/450) of hides and 27% (122/450) of carcasses were STEC PCR positive. Forty isolates representing 12 STEC serotypes (O5:H-,O13:H2, O26:H11, O33:H11, O55:H11, O113:H4, O128:H8, O136:H12, O138:H48, O150:H2, O168:H8 and ONT:H11) and 15 serotype-virulotype combinations were identified.This study provides much needed non-O157 STEC surveillance data and also provides further evidence of bovines as a source of clinically significant STEC as well as identifying 3 emerging serotypes O5:H- (eae-b1), O13:H2 (eae-z), and O150:H2 (eae-z) that should be considered when developing beef testing procedures for non-O157 STEC.
    LanguageEnglish
    Pages223-229
    JournalFood Microbiology
    Volume32
    Issue number2
    DOIs
    Publication statusPublished - Dec 2012

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    Shiga-Toxigenic Escherichia coli
    Shiga toxin-producing Escherichia coli
    serotypes
    cattle
    beef
    Polymerase Chain Reaction
    Abattoirs
    slaughterhouses
    Serogroup
    monitoring
    Red Meat
    animals

    Cite this

    Monaghan, A ; Byrne, B ; Fanning, S ; Sweeny, T ; McDowell, D.A. ; Bolton, D J. / Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses. In: Food Microbiology. 2012 ; Vol. 32, No. 2. pp. 223-229.
    @article{da498aa9f4fb49b4b37e9af18ac258be,
    title = "Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses",
    abstract = "Four hundred and fifty beef animal hides and a similar number of carcasses were screened for STEC in 3 beef abattoirs over a 12 month period using PCR and culture based methods. 67{\%} (301/450) of hides and 27{\%} (122/450) of carcasses were STEC PCR positive. Forty isolates representing 12 STEC serotypes (O5:H-,O13:H2, O26:H11, O33:H11, O55:H11, O113:H4, O128:H8, O136:H12, O138:H48, O150:H2, O168:H8 and ONT:H11) and 15 serotype-virulotype combinations were identified.This study provides much needed non-O157 STEC surveillance data and also provides further evidence of bovines as a source of clinically significant STEC as well as identifying 3 emerging serotypes O5:H- (eae-b1), O13:H2 (eae-z), and O150:H2 (eae-z) that should be considered when developing beef testing procedures for non-O157 STEC.",
    author = "A Monaghan and B Byrne and S Fanning and T Sweeny and D.A. McDowell and Bolton, {D J}",
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    year = "2012",
    month = "12",
    doi = "10.1016/j.fm.2012.06.002",
    language = "English",
    volume = "32",
    pages = "223--229",
    journal = "Food Microbiology",
    issn = "0740-0020",
    publisher = "Elsevier",
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    Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses. / Monaghan, A; Byrne, B; Fanning, S; Sweeny, T; McDowell, D.A.; Bolton, D J.

    In: Food Microbiology, Vol. 32, No. 2, 12.2012, p. 223-229.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses

    AU - Monaghan, A

    AU - Byrne, B

    AU - Fanning, S

    AU - Sweeny, T

    AU - McDowell, D.A.

    AU - Bolton, D J

    N1 - Reference text: Aidar-Ugrinovich, L., Blanco, J., Blanco, M., Blanco, J.E., Leomil, L., Dahbi, G., Mora, A., Onuma, D.L., Silveira, W.D., de Castro, A.F.P., 2007. Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in Sao Paulo, Brazil. International Journal of Food Microbiology 115, 297-306. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller,W., Lipman, D.J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389-3402. Anon, 1999. Zoonotic non-O157 Shiga toxin-producing Escherichia coli (STEC). Report of a WHO Scientific Working Group meeting, 23e26 June 1998, Berlin, Germany, pp. 1-30. Anon, 2007. Scientific opinion of the panel on biological hazards on a request from EFSA on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic types. 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    PY - 2012/12

    Y1 - 2012/12

    N2 - Four hundred and fifty beef animal hides and a similar number of carcasses were screened for STEC in 3 beef abattoirs over a 12 month period using PCR and culture based methods. 67% (301/450) of hides and 27% (122/450) of carcasses were STEC PCR positive. Forty isolates representing 12 STEC serotypes (O5:H-,O13:H2, O26:H11, O33:H11, O55:H11, O113:H4, O128:H8, O136:H12, O138:H48, O150:H2, O168:H8 and ONT:H11) and 15 serotype-virulotype combinations were identified.This study provides much needed non-O157 STEC surveillance data and also provides further evidence of bovines as a source of clinically significant STEC as well as identifying 3 emerging serotypes O5:H- (eae-b1), O13:H2 (eae-z), and O150:H2 (eae-z) that should be considered when developing beef testing procedures for non-O157 STEC.

    AB - Four hundred and fifty beef animal hides and a similar number of carcasses were screened for STEC in 3 beef abattoirs over a 12 month period using PCR and culture based methods. 67% (301/450) of hides and 27% (122/450) of carcasses were STEC PCR positive. Forty isolates representing 12 STEC serotypes (O5:H-,O13:H2, O26:H11, O33:H11, O55:H11, O113:H4, O128:H8, O136:H12, O138:H48, O150:H2, O168:H8 and ONT:H11) and 15 serotype-virulotype combinations were identified.This study provides much needed non-O157 STEC surveillance data and also provides further evidence of bovines as a source of clinically significant STEC as well as identifying 3 emerging serotypes O5:H- (eae-b1), O13:H2 (eae-z), and O150:H2 (eae-z) that should be considered when developing beef testing procedures for non-O157 STEC.

    U2 - 10.1016/j.fm.2012.06.002

    DO - 10.1016/j.fm.2012.06.002

    M3 - Article

    VL - 32

    SP - 223

    EP - 229

    JO - Food Microbiology

    T2 - Food Microbiology

    JF - Food Microbiology

    SN - 0740-0020

    IS - 2

    ER -