Sensitivity Enhancement of Point-of-Care for Cardiac Markers Detection using Micro-Impedimetric Immunosensor Arrays

Eyad M. Hamad, Samer I. Al-Gharabli, James A. McLaughlin

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    This paper describes the development and characterisation of a novel, electrical impedance spectroscopy-based (EIS) immunosensor array for point-of-care applications. EIS is a highly sensitive, label-free, real time technique suitable for single use, point-of-care cardiac marker detection devices. However, the underlying source of the observed change in EIS immunoassay response has not been well characterised or understood. A full understanding of the relationship between target binding and impedance response would significantly advance biosensor design and most probably increase detection limit sensitivity. The development of micro-/nano- structured electrodes for multi-frequency EIS procedure propose substantial benefits over classical macro-structured systems.Countless manipulations of electrode features and inter-electrode spacing will enhance the electrode surface area, increase the charge-transfer resistance and reduce the double-layer capacitance. These in turn give rise to improved signal-to-noise ratios, therefore affording greater sensitivity, lower detection limits and faster detection times.The sensor sensitivity range was within that required for human myoglobin determination, following acute myocardial infarction (heart attack). Real-time MyAb-MyAg interaction monitoring, permitted the determination of the binding events in less than one minute.

    Original languageEnglish
    Title of host publication2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
    PublisherInstitute of Electrical and Electronics Engineers Inc.
    Pages1119-1122
    Number of pages4
    ISBN (Electronic)9781538613115
    DOIs
    Publication statusPublished - Jul 2019
    Event41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019 - Berlin, Germany
    Duration: 23 Jul 201927 Jul 2019

    Publication series

    NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
    ISSN (Print)1557-170X

    Conference

    Conference41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
    CountryGermany
    CityBerlin
    Period23/07/1927/07/19

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