TY - GEN
T1 - Sensitivity Enhancement of Point-of-Care for Cardiac Markers Detection using Micro-Impedimetric Immunosensor Arrays
AU - Hamad, Eyad M.
AU - Al-Gharabli, Samer I.
AU - McLaughlin, James A.
N1 - Publisher Copyright:
© 2019 IEEE.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019/7
Y1 - 2019/7
N2 - This paper describes the development and characterisation of a novel, electrical impedance spectroscopy-based (EIS) immunosensor array for point-of-care applications. EIS is a highly sensitive, label-free, real time technique suitable for single use, point-of-care cardiac marker detection devices. However, the underlying source of the observed change in EIS immunoassay response has not been well characterised or understood. A full understanding of the relationship between target binding and impedance response would significantly advance biosensor design and most probably increase detection limit sensitivity. The development of micro-/nano- structured electrodes for multi-frequency EIS procedure propose substantial benefits over classical macro-structured systems.Countless manipulations of electrode features and inter-electrode spacing will enhance the electrode surface area, increase the charge-transfer resistance and reduce the double-layer capacitance. These in turn give rise to improved signal-to-noise ratios, therefore affording greater sensitivity, lower detection limits and faster detection times.The sensor sensitivity range was within that required for human myoglobin determination, following acute myocardial infarction (heart attack). Real-time MyAb-MyAg interaction monitoring, permitted the determination of the binding events in less than one minute.
AB - This paper describes the development and characterisation of a novel, electrical impedance spectroscopy-based (EIS) immunosensor array for point-of-care applications. EIS is a highly sensitive, label-free, real time technique suitable for single use, point-of-care cardiac marker detection devices. However, the underlying source of the observed change in EIS immunoassay response has not been well characterised or understood. A full understanding of the relationship between target binding and impedance response would significantly advance biosensor design and most probably increase detection limit sensitivity. The development of micro-/nano- structured electrodes for multi-frequency EIS procedure propose substantial benefits over classical macro-structured systems.Countless manipulations of electrode features and inter-electrode spacing will enhance the electrode surface area, increase the charge-transfer resistance and reduce the double-layer capacitance. These in turn give rise to improved signal-to-noise ratios, therefore affording greater sensitivity, lower detection limits and faster detection times.The sensor sensitivity range was within that required for human myoglobin determination, following acute myocardial infarction (heart attack). Real-time MyAb-MyAg interaction monitoring, permitted the determination of the binding events in less than one minute.
UR - http://www.scopus.com/inward/record.url?scp=85077876714&partnerID=8YFLogxK
U2 - 10.1109/EMBC.2019.8857546
DO - 10.1109/EMBC.2019.8857546
M3 - Conference contribution
C2 - 31946090
AN - SCOPUS:85077876714
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 1119
EP - 1122
BT - 2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
Y2 - 23 July 2019 through 27 July 2019
ER -