Self-assembled monolayers of alendronate on Ti6Al4V alloy surfaces enhance osteogenesis in mesenchymal stem cells

Luis Rojo, Borzo Gharibi, Robert McLister, Brian Meenan, Sanjukta Deb

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Phosphonates have emerged as an alternative for functionalization of titanium surfaces by theformation of homogeneous self-assembled monolayers (SAMs) via Ti-O-P linkages. This study presents results from an investigation of the modification of Ti6Al4V alloy by chemisorption of osseoinductive alendronate using a simple, effective and clean methodology. The modified surfaces showed a tailored topography and surface chemistry as determined by SEM microscopy and RAMAN spectroscopy. X-ray photoelectron spectroscopy revealed that an effective mode of bonding is created between the metal oxide surface and the phosphate residue of alendronate, leading to formation of homogenous drug distribution along the surface. In-vitro studies showed that alendronate SAMs induce differentiation of hMSC to a bone cell phenotype and promote bone formation on modified surfaces. Here we show that this novel method for the preparation of functional coatings on titanium-based medical devices provides osseoinductive bioactive molecules to promote enhanced integration at the site of implantation.
LanguageEnglish
Article number30548
Number of pages10
JournalScientific Reports
Volume6
Issue number30548
DOIs
Publication statusPublished - 29 Jul 2016

Fingerprint

Alendronate
Self assembled monolayers
Stem cells
Titanium
Bone
Organophosphonates
Chemisorption
Surface chemistry
Oxides
Topography
Microscopic examination
X ray photoelectron spectroscopy
Metals
Phosphates
titanium alloy (TiAl6V4)
Spectroscopy
Coatings
Scanning electron microscopy
Molecules
Pharmaceutical Preparations

Keywords

  • Phosphonates
  • titanium surfaces
  • self-assembled monolayers (SAMs)
  • alendronate
  • hMSC
  • bone formation.

Cite this

Rojo, Luis ; Gharibi, Borzo ; McLister, Robert ; Meenan, Brian ; Deb, Sanjukta. / Self-assembled monolayers of alendronate on Ti6Al4V alloy surfaces enhance osteogenesis in mesenchymal stem cells. In: Scientific Reports. 2016 ; Vol. 6, No. 30548.
@article{e9271791375a4097ba055353af393a97,
title = "Self-assembled monolayers of alendronate on Ti6Al4V alloy surfaces enhance osteogenesis in mesenchymal stem cells",
abstract = "Phosphonates have emerged as an alternative for functionalization of titanium surfaces by theformation of homogeneous self-assembled monolayers (SAMs) via Ti-O-P linkages. This study presents results from an investigation of the modification of Ti6Al4V alloy by chemisorption of osseoinductive alendronate using a simple, effective and clean methodology. The modified surfaces showed a tailored topography and surface chemistry as determined by SEM microscopy and RAMAN spectroscopy. X-ray photoelectron spectroscopy revealed that an effective mode of bonding is created between the metal oxide surface and the phosphate residue of alendronate, leading to formation of homogenous drug distribution along the surface. In-vitro studies showed that alendronate SAMs induce differentiation of hMSC to a bone cell phenotype and promote bone formation on modified surfaces. Here we show that this novel method for the preparation of functional coatings on titanium-based medical devices provides osseoinductive bioactive molecules to promote enhanced integration at the site of implantation.",
keywords = "Phosphonates, titanium surfaces, self-assembled monolayers (SAMs), alendronate, hMSC, bone formation.",
author = "Luis Rojo and Borzo Gharibi and Robert McLister and Brian Meenan and Sanjukta Deb",
year = "2016",
month = "7",
day = "29",
doi = "10.1038/srep30548",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
number = "30548",

}

Self-assembled monolayers of alendronate on Ti6Al4V alloy surfaces enhance osteogenesis in mesenchymal stem cells. / Rojo, Luis; Gharibi, Borzo; McLister, Robert; Meenan, Brian; Deb, Sanjukta.

In: Scientific Reports, Vol. 6, No. 30548, 30548, 29.07.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Self-assembled monolayers of alendronate on Ti6Al4V alloy surfaces enhance osteogenesis in mesenchymal stem cells

AU - Rojo, Luis

AU - Gharibi, Borzo

AU - McLister, Robert

AU - Meenan, Brian

AU - Deb, Sanjukta

PY - 2016/7/29

Y1 - 2016/7/29

N2 - Phosphonates have emerged as an alternative for functionalization of titanium surfaces by theformation of homogeneous self-assembled monolayers (SAMs) via Ti-O-P linkages. This study presents results from an investigation of the modification of Ti6Al4V alloy by chemisorption of osseoinductive alendronate using a simple, effective and clean methodology. The modified surfaces showed a tailored topography and surface chemistry as determined by SEM microscopy and RAMAN spectroscopy. X-ray photoelectron spectroscopy revealed that an effective mode of bonding is created between the metal oxide surface and the phosphate residue of alendronate, leading to formation of homogenous drug distribution along the surface. In-vitro studies showed that alendronate SAMs induce differentiation of hMSC to a bone cell phenotype and promote bone formation on modified surfaces. Here we show that this novel method for the preparation of functional coatings on titanium-based medical devices provides osseoinductive bioactive molecules to promote enhanced integration at the site of implantation.

AB - Phosphonates have emerged as an alternative for functionalization of titanium surfaces by theformation of homogeneous self-assembled monolayers (SAMs) via Ti-O-P linkages. This study presents results from an investigation of the modification of Ti6Al4V alloy by chemisorption of osseoinductive alendronate using a simple, effective and clean methodology. The modified surfaces showed a tailored topography and surface chemistry as determined by SEM microscopy and RAMAN spectroscopy. X-ray photoelectron spectroscopy revealed that an effective mode of bonding is created between the metal oxide surface and the phosphate residue of alendronate, leading to formation of homogenous drug distribution along the surface. In-vitro studies showed that alendronate SAMs induce differentiation of hMSC to a bone cell phenotype and promote bone formation on modified surfaces. Here we show that this novel method for the preparation of functional coatings on titanium-based medical devices provides osseoinductive bioactive molecules to promote enhanced integration at the site of implantation.

KW - Phosphonates

KW - titanium surfaces

KW - self-assembled monolayers (SAMs)

KW - alendronate

KW - hMSC

KW - bone formation.

UR - https://pure.ulster.ac.uk/en/publications/self-assembled-monolayers-of-alendronate-on-ti6al4v-alloy-surface-3

U2 - 10.1038/srep30548

DO - 10.1038/srep30548

M3 - Article

VL - 6

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 30548

M1 - 30548

ER -