Selenium Yeast Dietary Supplement Affects Rumen Bacterial Population Dynamics and Fermentation Parameters of Tibetan Sheep (

Xiongxiong Cui, Zhaofeng Wang, Yuhui Tan, Shenghua Chang, Huiru Zheng, Haiying Wang, Tianhai Yan, Tsedan Guru, Fujiang Hou

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Abstract

Selenium (Se) deficiency is a widespread and seasonally chronic phenomenon observed in Tibetan sheep ( ) traditionally grazed on the Qinghai-Tibet Plateau (QTP). Effects of the dietary addition of Se-enriched yeast (SeY) on the bacterial community in sheep rumen and rumen fermentation were evaluated with the aim of gaining a better understanding of the rumen prokaryotic community. Twenty-four yearling Tibetan rams [initial average body weight (BW) of 31.0 ± 0.64 kg] were randomly divided into four treatment groups, namely, control (CK), low Se (L), medium Se (M), and high Se (H). Each group comprised six rams and was fed a basic diet of fresh forage cut from the alpine meadow, to which SeY was added at prescribed dose rates. This feed trial was conducted for over 35 days. On the final day, rumen fluid was collected using a transesophageal sampler for analyzing rumen pH, NH -N content, volatile fatty acid (VFA) level, and the rumen microbial community. Our analyses showed that NH -N, total VFA, and propionate concentrations in the M group were significantly higher than in the other groups ( < 0.05). Both the principal coordinates analysis (PCoA) and the analysis of similarities revealed that the bacterial population structure of rumen differed among the four groups. The predominant rumen bacterial phyla were found to be Bacteroidetes and Firmicutes, and the three dominant genera in all the samples across all treatments were R7 group, RC9 gut group, and 1. The relative abundances of 1, RC9 gut group, 2, XPB1014 group, , and Hafnia- were found to differ significantly among the four treatment groups ( < 0.05). Moreover, Tax4fun metagenome estimation revealed that gene functions and metabolic pathways associated with carbohydrate and other amino acids were overexpressed in the rumen microbiota of SeY-supplemented sheep. To conclude, SeY significantly affects the abundance of rumen bacteria and ultimately affects the rumen microbial fermentation. [Abstract copyright: Copyright © 2021 Cui, Wang, Tan, Chang, Zheng, Wang, Yan, Guru and Hou.]
Original languageEnglish
Article number663945
JournalFrontiers in Microbiology
Volume12
Early online date2 Jul 2021
DOIs
Publication statusE-pub ahead of print - 2 Jul 2021

Keywords

  • Qinghai-Tibet plateau
  • grazing
  • rumen bacterial communities
  • selenium
  • alpine meadow selenium
  • high-throughput sequencing
  • bacterial communities
  • Tibetan sheep

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