TY - JOUR
T1 - Screening of Bacterial Isolates from Seafood-Wastes for Chitin Degrading Enzyme Activity
AU - Thomas, Starlin
AU - Patil, Ashish B.
AU - Salgaonkar, Prathamesh N.
AU - Shrivastava, Smriti
AU - Singh - Nee Nigam, Poonam
PY - 2020/5/18
Y1 - 2020/5/18
N2 - The safer removal of chitin is important for controlling accumulation of chitin in environment and agriculture. Chitin a long-chain polymer is a glucosederivative and a primary component of fungal cell-wall, the exoskeleton of molluscs, fish, and insects. Chitinase enzyme (E.C. 3.2.1.14) is able to digest chitin, hydrolyzing the 1-4 linkages between the N-acetyleglucosamine molecules. Present work was undertaken for the isolation and characterisation of chitinolytic bacteria from waste-seafood. Seventy-one indigenous strains were isolated from a variety of marine-sources, including scales of sea-fish, shrimps, prawns and crabs; of which 4isolates were identified as Alcaligenes spp., 2as Kurthiaspp., 1 as Acinetobactor and 1 as Bacillus spp. These were higher chitinase producers with enzyme units ranging from 12.5 to 35.41 micromoles/min/ml. These chitinase producers may prove to be industrially beneficial for their enzyme production for degradation of chitin in environment clean-up, polluted with decaying sea-food and wastes originated from their processing.
AB - The safer removal of chitin is important for controlling accumulation of chitin in environment and agriculture. Chitin a long-chain polymer is a glucosederivative and a primary component of fungal cell-wall, the exoskeleton of molluscs, fish, and insects. Chitinase enzyme (E.C. 3.2.1.14) is able to digest chitin, hydrolyzing the 1-4 linkages between the N-acetyleglucosamine molecules. Present work was undertaken for the isolation and characterisation of chitinolytic bacteria from waste-seafood. Seventy-one indigenous strains were isolated from a variety of marine-sources, including scales of sea-fish, shrimps, prawns and crabs; of which 4isolates were identified as Alcaligenes spp., 2as Kurthiaspp., 1 as Acinetobactor and 1 as Bacillus spp. These were higher chitinase producers with enzyme units ranging from 12.5 to 35.41 micromoles/min/ml. These chitinase producers may prove to be industrially beneficial for their enzyme production for degradation of chitin in environment clean-up, polluted with decaying sea-food and wastes originated from their processing.
KW - Chitinase
KW - Chitin
KW - Bacteria
KW - enzyme activity
KW - Sea wastes
M3 - Article
SN - 2333-6633
VL - 5
SP - 1
EP - 8
JO - Chemical Engineering & Process Techniques
JF - Chemical Engineering & Process Techniques
IS - 1
M1 - 1059
ER -