Salmonella enterica var Typhimurium and Salmonella enterica var Enteritidis express type 1 fimbriae in the rat in vivo

SWB Ewen, Patrick Naughton, G Grant, M Sojka, E AllenVercoe, S Bardocz, CJ Thorns, A Pusztai

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In a series of experiments rats were dosed with purified type 1 fimbriae from Salmonella enterica var Enteritidis or with fimbriated cultures of either S. enterica var Typhimurium or S. enterica var Enteritidis, Paraffin-wax embedded histological sections of jejunal and ileal tissue were taken and stained by the streptavidin biotin complex (sABC) staining technique for the detection of salmonella and type 1 fimbriae. On oral infection with Enteritidis and Typhimurium both bacteria were shown to be closely associated with the rat ileal epithelium and expressed type 1 fimbriae, thus clearly demonstrating that type 1 fimbriae are expressed by salmonellae in vivo. Moreover, association with the ileum was also shown to occur when purified type 1 fimbriae were orally administered to rats. Our results suggest that type 1 fimbriae alone or in combination with other fimbriae may play an important role in the early stages of infection with these pathogenic bacteria.
LanguageEnglish
Pages185-192
JournalFEMS Immunology and Medical Microbiology
Volume18
Issue number3
Publication statusPublished - Jul 1997

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Salmonella enterica
Salmonella
Bacteria
Waxes
Infection
Ileum
Paraffin
Epithelium
Staining and Labeling

Keywords

  • Rat model
  • Salmonella
  • Type 1 fimbriae

Cite this

Ewen, SWB ; Naughton, Patrick ; Grant, G ; Sojka, M ; AllenVercoe, E ; Bardocz, S ; Thorns, CJ ; Pusztai, A. / Salmonella enterica var Typhimurium and Salmonella enterica var Enteritidis express type 1 fimbriae in the rat in vivo. In: FEMS Immunology and Medical Microbiology. 1997 ; Vol. 18, No. 3. pp. 185-192.
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abstract = "In a series of experiments rats were dosed with purified type 1 fimbriae from Salmonella enterica var Enteritidis or with fimbriated cultures of either S. enterica var Typhimurium or S. enterica var Enteritidis, Paraffin-wax embedded histological sections of jejunal and ileal tissue were taken and stained by the streptavidin biotin complex (sABC) staining technique for the detection of salmonella and type 1 fimbriae. On oral infection with Enteritidis and Typhimurium both bacteria were shown to be closely associated with the rat ileal epithelium and expressed type 1 fimbriae, thus clearly demonstrating that type 1 fimbriae are expressed by salmonellae in vivo. Moreover, association with the ileum was also shown to occur when purified type 1 fimbriae were orally administered to rats. Our results suggest that type 1 fimbriae alone or in combination with other fimbriae may play an important role in the early stages of infection with these pathogenic bacteria.",
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Ewen, SWB, Naughton, P, Grant, G, Sojka, M, AllenVercoe, E, Bardocz, S, Thorns, CJ & Pusztai, A 1997, 'Salmonella enterica var Typhimurium and Salmonella enterica var Enteritidis express type 1 fimbriae in the rat in vivo', FEMS Immunology and Medical Microbiology, vol. 18, no. 3, pp. 185-192.

Salmonella enterica var Typhimurium and Salmonella enterica var Enteritidis express type 1 fimbriae in the rat in vivo. / Ewen, SWB; Naughton, Patrick; Grant, G; Sojka, M; AllenVercoe, E; Bardocz, S; Thorns, CJ; Pusztai, A.

In: FEMS Immunology and Medical Microbiology, Vol. 18, No. 3, 07.1997, p. 185-192.

Research output: Contribution to journalArticle

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AU - Ewen, SWB

AU - Naughton, Patrick

AU - Grant, G

AU - Sojka, M

AU - AllenVercoe, E

AU - Bardocz, S

AU - Thorns, CJ

AU - Pusztai, A

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AB - In a series of experiments rats were dosed with purified type 1 fimbriae from Salmonella enterica var Enteritidis or with fimbriated cultures of either S. enterica var Typhimurium or S. enterica var Enteritidis, Paraffin-wax embedded histological sections of jejunal and ileal tissue were taken and stained by the streptavidin biotin complex (sABC) staining technique for the detection of salmonella and type 1 fimbriae. On oral infection with Enteritidis and Typhimurium both bacteria were shown to be closely associated with the rat ileal epithelium and expressed type 1 fimbriae, thus clearly demonstrating that type 1 fimbriae are expressed by salmonellae in vivo. Moreover, association with the ileum was also shown to occur when purified type 1 fimbriae were orally administered to rats. Our results suggest that type 1 fimbriae alone or in combination with other fimbriae may play an important role in the early stages of infection with these pathogenic bacteria.

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