Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection

Alif Chebbi, Mohamed Elshikh, Farazul Haque, Syed Ahmed, Sara Dobbin, R Marchant, Sami Sayadi, Mohamed Chamkha, Ibrahim M Banat

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L−1 and a critical micelle concentration (CMC) of 80 mg L−1. P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0.1, 0.5, and 1 mg ml−1 on a stainless steel surface commonly used in industrial process pipelines. CFU measurements and LIVE/DEAD BacLight staining confirmed these observations. Furthermore, a purified di-rhamnolipid fraction was found to be responsible for the microbial inhibition of B. licheniformis strain CAN55. This study providesevidence that rhamnolipids may have valuable applications in preventing biofilms and biofouling in industrial plants and, in a wider context, may also apply to metal medical devices.
LanguageEnglish
Pages1-12
JournalJOURNAL OF BASIC MICROBIOLOGY
Volume9999
Early online date3 Feb 2017
DOIs
Publication statusPublished - 2 May 2017

Fingerprint

biofouling
biofilm
metal
microorganism
steel
fluid
product
flowing water
organism
industrial process

Keywords

  • biofilm inhibition
  • biofouling
  • biosurfactants
  • rhamnolipids
  • metal surface

Cite this

Chebbi, Alif ; Elshikh, Mohamed ; Haque, Farazul ; Ahmed, Syed ; Dobbin, Sara ; Marchant, R ; Sayadi, Sami ; Chamkha, Mohamed ; Banat, Ibrahim M. / Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection. In: JOURNAL OF BASIC MICROBIOLOGY. 2017 ; Vol. 9999. pp. 1-12.
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abstract = "Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L−1 and a critical micelle concentration (CMC) of 80 mg L−1. P. aeruginosa W10 rhamnolipids were able to disrupt up to 99{\%} of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0.1, 0.5, and 1 mg ml−1 on a stainless steel surface commonly used in industrial process pipelines. CFU measurements and LIVE/DEAD BacLight staining confirmed these observations. Furthermore, a purified di-rhamnolipid fraction was found to be responsible for the microbial inhibition of B. licheniformis strain CAN55. This study providesevidence that rhamnolipids may have valuable applications in preventing biofilms and biofouling in industrial plants and, in a wider context, may also apply to metal medical devices.",
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Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection. / Chebbi, Alif; Elshikh, Mohamed; Haque, Farazul; Ahmed, Syed; Dobbin, Sara; Marchant, R; Sayadi, Sami; Chamkha, Mohamed; Banat, Ibrahim M.

In: JOURNAL OF BASIC MICROBIOLOGY, Vol. 9999, 02.05.2017, p. 1-12.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection

AU - Chebbi, Alif

AU - Elshikh, Mohamed

AU - Haque, Farazul

AU - Ahmed, Syed

AU - Dobbin, Sara

AU - Marchant, R

AU - Sayadi, Sami

AU - Chamkha, Mohamed

AU - Banat, Ibrahim M

PY - 2017/5/2

Y1 - 2017/5/2

N2 - Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L−1 and a critical micelle concentration (CMC) of 80 mg L−1. P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0.1, 0.5, and 1 mg ml−1 on a stainless steel surface commonly used in industrial process pipelines. CFU measurements and LIVE/DEAD BacLight staining confirmed these observations. Furthermore, a purified di-rhamnolipid fraction was found to be responsible for the microbial inhibition of B. licheniformis strain CAN55. This study providesevidence that rhamnolipids may have valuable applications in preventing biofilms and biofouling in industrial plants and, in a wider context, may also apply to metal medical devices.

AB - Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L−1 and a critical micelle concentration (CMC) of 80 mg L−1. P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0.1, 0.5, and 1 mg ml−1 on a stainless steel surface commonly used in industrial process pipelines. CFU measurements and LIVE/DEAD BacLight staining confirmed these observations. Furthermore, a purified di-rhamnolipid fraction was found to be responsible for the microbial inhibition of B. licheniformis strain CAN55. This study providesevidence that rhamnolipids may have valuable applications in preventing biofilms and biofouling in industrial plants and, in a wider context, may also apply to metal medical devices.

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KW - biofouling

KW - biosurfactants

KW - rhamnolipids

KW - metal surface

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