Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens

Mohamed Elshikh, Scott Funston, Alif chebbi, Syed Ahmed, R Marchant, Ibrahim M Banat

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa. In this article, we have reported the production and characterisation of long chain rhamnolipids from non-pathogenic Burkholderia thailandensis E264 (ATCC 700388). We have also investigated the antibacterial and antibiofilm properties of these rhamnolipids against some oral pathogens (Streptococcus oralis, Actinomyces naeslundii, Neisseria mucosa and Streptococcus sanguinis), important for oral health and hygiene. Treating these bacteria with different concentrations of long chain rhamnolipids resulted in a reduction of 3–4 log of bacterial viability, placing these rhamnolipids close to being classified as biocidal. Investigating long chain rhamnolipid efficacy as antibiofilm agents for prospective oral-related applications revealed good potency against oral-bacteria biofilms in a co-incubation experiments, in a pre-coated surface format, in disrupting immature biofilms and has shown excellent combination effect with Lauryl Sodium Sulphate which resulted in a drastic decrease in its minimal inhibitory concentration against different bacteria. Investigating the rhamnolipid permeabilization effect along with their ability to induce the formation of reactive oxygen species has shed light on the mechanism through which inhibition/killing of bacteria may occur.
LanguageEnglish
Pages26-36
JournalNew Biotechnology
Volume36
DOIs
Publication statusPublished - 5 Jan 2017

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Pathogens
Bacteria
Biofilms
Consumer products
rhamnolipid
Sodium Dodecyl Sulfate
Reactive Oxygen Species
Health

Keywords

  • Long chain rhamnolipids
  • Non-pathogenic bacteria
  • Biofilm
  • Oral pathogens

Cite this

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Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens. / Elshikh, Mohamed; Funston, Scott; chebbi, Alif; Ahmed, Syed; Marchant, R; Banat, Ibrahim M.

In: New Biotechnology, Vol. 36, 05.01.2017, p. 26-36.

Research output: Contribution to journalArticle

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T1 - Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens

AU - Elshikh, Mohamed

AU - Funston, Scott

AU - chebbi, Alif

AU - Ahmed, Syed

AU - Marchant, R

AU - Banat, Ibrahim M

PY - 2017/1/5

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