Rhamnolipids and nutrients boost remediation of crude oil contaminated soil by enhancing bacterial colonization and metabolic activities

Razia Tahseen, Muhammad Afzal, Samina Iqbal, Ghulam Shabir, Qaiser M. Khan, Zafar M. Khalid, Ibrahim M Banat

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bioaugmentation is a promising approach for the remediation of soil and water contaminated with petroleum hydrocarbons. Addition of rhamnolipids and nutrients in the soil enhance survival and metabolic activity of microbes and thus has the potential to restore the quality of soil to a greater extent. This study was carried out to evaluate the prospective role that these supplementary compounds can play in remediation of crude oil-contaminated soil by aiding bacterial strains to survive and perform better. A consortium of hydrocarbon degrading bacteria was inoculated in crude oil-contaminated soil and supplemented with different combinations of rhamnolipids and nutrients. Maximum crude oil degradation of 77.6% was observed in the soil inoculated with hydrocarbon-degrading bacteria supplemented with rhamnolipids and nutrients. Moreover, addition of supplementary compounds enhanced bacterial survival as well as abundance and expression of alkane hydroxylase gene, alkB, in oil contaminated soil. A strong positive relationship (r ¼ 0.94) observed between gene expression and crude oil reduction indicates that catabolic gene expression is essential for hydrocarbon mineralization. All results from the investigation collectively confirm that addition of rhamnolipids and nutrients enhance bacterial colonization and metabolic activity thus improving remediation of crude oil contaminated soil.
LanguageEnglish
Pages192-198
JournalInternational Biodeterioration & Biodegradation
Volume115
Early online date7 Sep 2017
DOIs
Publication statusE-pub ahead of print - 7 Sep 2017

Fingerprint

Petroleum
Remediation
Nutrients
crude oil
remediation
Soil
colonization
Crude oil
Soils
Food
nutrient
Hydrocarbons
hydrocarbon
gene expression
soil
Gene expression
bacterium
petroleum hydrocarbon
Bacteria
Cytochrome P-450 CYP4A

Keywords

  • Crude oil
  • Bioremediation
  • Rhamnolipids
  • Nutrients
  • Gene abundance
  • Gene expression

Cite this

Tahseen, Razia ; Afzal, Muhammad ; Iqbal, Samina ; Shabir, Ghulam ; Khan, Qaiser M. ; Khalid, Zafar M. ; Banat, Ibrahim M. / Rhamnolipids and nutrients boost remediation of crude oil contaminated soil by enhancing bacterial colonization and metabolic activities. In: International Biodeterioration & Biodegradation. 2017 ; Vol. 115. pp. 192-198.
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Rhamnolipids and nutrients boost remediation of crude oil contaminated soil by enhancing bacterial colonization and metabolic activities. / Tahseen, Razia; Afzal, Muhammad; Iqbal, Samina; Shabir, Ghulam; Khan, Qaiser M.; Khalid, Zafar M.; Banat, Ibrahim M.

In: International Biodeterioration & Biodegradation, Vol. 115, 07.09.2017, p. 192-198.

Research output: Contribution to journalArticle

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