Rhamnolipids and Lactonic Sophorolipids: Natural antimicrobial surfactants for oral hygiene

M Elshikh, I Moya-Ramirez, H Moens, S Roelants, W Soetaert, Roger Marchant, Ibrahim M Banat

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aims: To assess the efficacy of rhamnolipid (mixture of mono- and di-rhamnolipid congeners, purified monorhamnolipid, dirhamnolipid and lactonic sophorolipid biosurfactants against pathogens important for oral hygiene.Methods and Results: Acquired and produced biosurfactants were fully characterised to allow the antimicrobial activity to be assigned to the biosurfactant congeners. Antimicrobial activity was assessed using the resazurin aided microdilution method. Mixed rhamnolipid JBR425 (MR) and lactonic sophorolipids demonstrated the lowest Minimum Inhibitory Concentration (MIC) which ranged between 100-400 µg ml-1 against Streptococcus mutans, S. oralis, Actinomyces naeslundii, Neisseria mucosa and S. sanguinis. Combining these biosurfactants with standard antimicrobial agents namely Chlorhexidine, Sodium Lauryl Sulphate, Tetracycline HCl and Ciprofloxacin showed a dramatic drop in the MIC values. In addition, in vitro studies demonstrated the biosurfactants’ ability to prevent and disrupt oral pathogens biofilms. The increased permeability of microorganisms treated with biosurfactant, as shown using Bisbenzimide dye, in part explains the inhibition effect.Conclusions: The results demonstrate that rhamnolipids and lactonic sophorolipids have the ability to inhibit oral pathogens both in planktonic and oral biofilm states.Significance and Impact of the Study: The findings indicate the potential value of biosurfactants for both oral hygiene and the pharmaceutical industries since there is a serious need to reduce the reliance on synthetic antimicrobials and antibiotics.
LanguageEnglish
JournalJournal of Applied Microbiology
Volume123
DOIs
Publication statusAccepted/In press - 28 Jul 2017

Fingerprint

Oral Hygiene
Surface-Active Agents
Microbial Sensitivity Tests
Biofilms
Neisseria mucosa
Bisbenzimidazole
Actinomyces
Chlorhexidine
Streptococcus mutans
Drug Industry
Ciprofloxacin
Anti-Infective Agents
Tetracycline
Sodium Dodecyl Sulfate
Permeability
Coloring Agents
Anti-Bacterial Agents
rhamnolipid
16-(2'-O-glucopyranosylglucopyranosyloxy)hexadecanoic acid 1',4''-lactone 6',6''-diacetate

Keywords

  • Rhamnolipids
  • Lactonic sophorolipids
  • Minimum Inhibitory Concentration
  • Biofilm
  • Oral hygiene.

Cite this

@article{e11d7346b2a641d0b22d12078e547857,
title = "Rhamnolipids and Lactonic Sophorolipids: Natural antimicrobial surfactants for oral hygiene",
abstract = "Aims: To assess the efficacy of rhamnolipid (mixture of mono- and di-rhamnolipid congeners, purified monorhamnolipid, dirhamnolipid and lactonic sophorolipid biosurfactants against pathogens important for oral hygiene.Methods and Results: Acquired and produced biosurfactants were fully characterised to allow the antimicrobial activity to be assigned to the biosurfactant congeners. Antimicrobial activity was assessed using the resazurin aided microdilution method. Mixed rhamnolipid JBR425 (MR) and lactonic sophorolipids demonstrated the lowest Minimum Inhibitory Concentration (MIC) which ranged between 100-400 µg ml-1 against Streptococcus mutans, S. oralis, Actinomyces naeslundii, Neisseria mucosa and S. sanguinis. Combining these biosurfactants with standard antimicrobial agents namely Chlorhexidine, Sodium Lauryl Sulphate, Tetracycline HCl and Ciprofloxacin showed a dramatic drop in the MIC values. In addition, in vitro studies demonstrated the biosurfactants’ ability to prevent and disrupt oral pathogens biofilms. The increased permeability of microorganisms treated with biosurfactant, as shown using Bisbenzimide dye, in part explains the inhibition effect.Conclusions: The results demonstrate that rhamnolipids and lactonic sophorolipids have the ability to inhibit oral pathogens both in planktonic and oral biofilm states.Significance and Impact of the Study: The findings indicate the potential value of biosurfactants for both oral hygiene and the pharmaceutical industries since there is a serious need to reduce the reliance on synthetic antimicrobials and antibiotics.",
keywords = "Rhamnolipids, Lactonic sophorolipids, Minimum Inhibitory Concentration, Biofilm, Oral hygiene.",
author = "M Elshikh and I Moya-Ramirez and H Moens and S Roelants and W Soetaert and Roger Marchant and Banat, {Ibrahim M}",
year = "2017",
month = "7",
day = "28",
doi = "10.1111/jam.13550",
language = "English",
volume = "123",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",

}

Rhamnolipids and Lactonic Sophorolipids: Natural antimicrobial surfactants for oral hygiene. / Elshikh, M; Moya-Ramirez, I; Moens, H; Roelants, S; Soetaert, W; Marchant, Roger; Banat, Ibrahim M.

In: Journal of Applied Microbiology, Vol. 123, 28.07.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rhamnolipids and Lactonic Sophorolipids: Natural antimicrobial surfactants for oral hygiene

AU - Elshikh, M

AU - Moya-Ramirez, I

AU - Moens, H

AU - Roelants, S

AU - Soetaert, W

AU - Marchant, Roger

AU - Banat, Ibrahim M

PY - 2017/7/28

Y1 - 2017/7/28

N2 - Aims: To assess the efficacy of rhamnolipid (mixture of mono- and di-rhamnolipid congeners, purified monorhamnolipid, dirhamnolipid and lactonic sophorolipid biosurfactants against pathogens important for oral hygiene.Methods and Results: Acquired and produced biosurfactants were fully characterised to allow the antimicrobial activity to be assigned to the biosurfactant congeners. Antimicrobial activity was assessed using the resazurin aided microdilution method. Mixed rhamnolipid JBR425 (MR) and lactonic sophorolipids demonstrated the lowest Minimum Inhibitory Concentration (MIC) which ranged between 100-400 µg ml-1 against Streptococcus mutans, S. oralis, Actinomyces naeslundii, Neisseria mucosa and S. sanguinis. Combining these biosurfactants with standard antimicrobial agents namely Chlorhexidine, Sodium Lauryl Sulphate, Tetracycline HCl and Ciprofloxacin showed a dramatic drop in the MIC values. In addition, in vitro studies demonstrated the biosurfactants’ ability to prevent and disrupt oral pathogens biofilms. The increased permeability of microorganisms treated with biosurfactant, as shown using Bisbenzimide dye, in part explains the inhibition effect.Conclusions: The results demonstrate that rhamnolipids and lactonic sophorolipids have the ability to inhibit oral pathogens both in planktonic and oral biofilm states.Significance and Impact of the Study: The findings indicate the potential value of biosurfactants for both oral hygiene and the pharmaceutical industries since there is a serious need to reduce the reliance on synthetic antimicrobials and antibiotics.

AB - Aims: To assess the efficacy of rhamnolipid (mixture of mono- and di-rhamnolipid congeners, purified monorhamnolipid, dirhamnolipid and lactonic sophorolipid biosurfactants against pathogens important for oral hygiene.Methods and Results: Acquired and produced biosurfactants were fully characterised to allow the antimicrobial activity to be assigned to the biosurfactant congeners. Antimicrobial activity was assessed using the resazurin aided microdilution method. Mixed rhamnolipid JBR425 (MR) and lactonic sophorolipids demonstrated the lowest Minimum Inhibitory Concentration (MIC) which ranged between 100-400 µg ml-1 against Streptococcus mutans, S. oralis, Actinomyces naeslundii, Neisseria mucosa and S. sanguinis. Combining these biosurfactants with standard antimicrobial agents namely Chlorhexidine, Sodium Lauryl Sulphate, Tetracycline HCl and Ciprofloxacin showed a dramatic drop in the MIC values. In addition, in vitro studies demonstrated the biosurfactants’ ability to prevent and disrupt oral pathogens biofilms. The increased permeability of microorganisms treated with biosurfactant, as shown using Bisbenzimide dye, in part explains the inhibition effect.Conclusions: The results demonstrate that rhamnolipids and lactonic sophorolipids have the ability to inhibit oral pathogens both in planktonic and oral biofilm states.Significance and Impact of the Study: The findings indicate the potential value of biosurfactants for both oral hygiene and the pharmaceutical industries since there is a serious need to reduce the reliance on synthetic antimicrobials and antibiotics.

KW - Rhamnolipids

KW - Lactonic sophorolipids

KW - Minimum Inhibitory Concentration

KW - Biofilm

KW - Oral hygiene.

U2 - 10.1111/jam.13550

DO - 10.1111/jam.13550

M3 - Article

VL - 123

JO - Journal of Applied Microbiology

T2 - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

ER -