Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials

K. S. Rahman, T. J. Rahman, Stephen McClean, R Marchant, Ibrahim Banat

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybean oil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C(10)-C(10)). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa GS9-119 emulsified only hexane and kerosene to that level.
LanguageEnglish
Pages1277-1281
JournalBiotechnology Progress
Volume18
Issue number6
Publication statusPublished - Nov 2002

Fingerprint

biosurfactants
Pseudomonas aeruginosa
raw materials
kerosene
hexane
xylene
oils
safflower oil
gasoline
emulsifying
benzene
soybean oil
hydrocarbons
glycerol
rhamnolipids
mass spectrometry

Keywords

  • Emulsions
  • Glycerol/metabolism
  • Glycolipids/biosynthesis/chemistry/economics
  • Hydrocarbons
  • Mass Spectrometry
  • Pseudomonas aeruginosa/chemistry/metabolism
  • Safflower Oil/metabolism
  • Soybean Oil/metabolism
  • Surface-Active Agents/economics

Cite this

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title = "Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials",
abstract = "This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybean oil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C(10)-C(10)). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70{\%} using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa GS9-119 emulsified only hexane and kerosene to that level.",
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Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials. / Rahman, K. S.; Rahman, T. J.; McClean, Stephen; Marchant, R; Banat, Ibrahim.

In: Biotechnology Progress, Vol. 18, No. 6, 11.2002, p. 1277-1281.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials

AU - Rahman, K. S.

AU - Rahman, T. J.

AU - McClean, Stephen

AU - Marchant, R

AU - Banat, Ibrahim

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N2 - This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybean oil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C(10)-C(10)). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa GS9-119 emulsified only hexane and kerosene to that level.

AB - This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybean oil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C(10)-C(10)). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa GS9-119 emulsified only hexane and kerosene to that level.

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KW - Glycolipids/biosynthesis/chemistry/economics

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KW - Mass Spectrometry

KW - Pseudomonas aeruginosa/chemistry/metabolism

KW - Safflower Oil/metabolism

KW - Soybean Oil/metabolism

KW - Surface-Active Agents/economics

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