Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation

O Al-Assar, T Robson, Stephanie McKeown, I Gardin, GD Wilson, DG Hirst

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    Abstract

    We irradiated different cellular compartments and measured changes in expression of the FOS gene at the mRNA and protein levels. [H-3]Thymidine and tritiated water were used to irradiate the nucleus and the whole cell, respectively. I-125-Concanavalin A binding was used to irradiate the cell membrane differentially. Changes in FOS mRNA and protein levels were measured using semi-quantitative RT-PCR and SDS-PAGE Western blotting, respectively, Irradiation of the nucleus or the whole cell at a dose rate of 0.075 Gy/h caused no change in the level of FOS mRNA expression, but modestly (1.5-fold) induced FOS protein after 0.5 h, Irradiation of the nucleus at a dose rate of 0.43 Gy/h induced FOS mRNA by 1.5-fold after 0.5 h, but there was no significant effect after whole-cell irradiation. FOS protein was transiently induced 2.5-fold above control levels 0.5 h after a 0.43-Gy/h exposure of the nucleus or the whole cell. Irradiation of the cell membrane at a dose rate of 1.8 Gy/h for up to 2 h caused no change in the levels of expression of FOS mRNA or protein, but a dose rate of 6.8 Gy/h transiently increased the level of FOS mRNA S-fold after 0.5 h, These data demonstrate the complexity of the cellular response to radiation-induced damage at low doses. The lack of quantitative agreement between the transcript and protein levels for FOS suggests a role for posttranscriptional regulation. (C) 2000 by Radiation Research Society.
    Original languageEnglish
    Pages (from-to)503-514
    JournalRadiation Research
    Volume154
    Issue number5
    Publication statusPublished - Nov 2000

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  • Cite this

    Al-Assar, O., Robson, T., McKeown, S., Gardin, I., Wilson, GD., & Hirst, DG. (2000). Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation. Radiation Research, 154(5), 503-514.