Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation

O Al-Assar, T Robson, Stephanie McKeown, I Gardin, GD Wilson, DG Hirst

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    We irradiated different cellular compartments and measured changes in expression of the FOS gene at the mRNA and protein levels. [H-3]Thymidine and tritiated water were used to irradiate the nucleus and the whole cell, respectively. I-125-Concanavalin A binding was used to irradiate the cell membrane differentially. Changes in FOS mRNA and protein levels were measured using semi-quantitative RT-PCR and SDS-PAGE Western blotting, respectively, Irradiation of the nucleus or the whole cell at a dose rate of 0.075 Gy/h caused no change in the level of FOS mRNA expression, but modestly (1.5-fold) induced FOS protein after 0.5 h, Irradiation of the nucleus at a dose rate of 0.43 Gy/h induced FOS mRNA by 1.5-fold after 0.5 h, but there was no significant effect after whole-cell irradiation. FOS protein was transiently induced 2.5-fold above control levels 0.5 h after a 0.43-Gy/h exposure of the nucleus or the whole cell. Irradiation of the cell membrane at a dose rate of 1.8 Gy/h for up to 2 h caused no change in the levels of expression of FOS mRNA or protein, but a dose rate of 6.8 Gy/h transiently increased the level of FOS mRNA S-fold after 0.5 h, These data demonstrate the complexity of the cellular response to radiation-induced damage at low doses. The lack of quantitative agreement between the transcript and protein levels for FOS suggests a role for posttranscriptional regulation. (C) 2000 by Radiation Research Society.
    LanguageEnglish
    Pages503-514
    JournalRadiation Research
    Volume154
    Issue number5
    Publication statusPublished - Nov 2000

    Fingerprint

    ionizing radiation
    irradiation
    dosage
    proteins
    cell membranes
    cells
    tritiated water
    concanavalin A
    thymidine
    Western blotting
    reverse transcriptase polymerase chain reaction
    gene expression

    Cite this

    Al-Assar, O., Robson, T., McKeown, S., Gardin, I., Wilson, GD., & Hirst, DG. (2000). Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation. Radiation Research, 154(5), 503-514.
    Al-Assar, O ; Robson, T ; McKeown, Stephanie ; Gardin, I ; Wilson, GD ; Hirst, DG. / Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation. In: Radiation Research. 2000 ; Vol. 154, No. 5. pp. 503-514.
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    abstract = "We irradiated different cellular compartments and measured changes in expression of the FOS gene at the mRNA and protein levels. [H-3]Thymidine and tritiated water were used to irradiate the nucleus and the whole cell, respectively. I-125-Concanavalin A binding was used to irradiate the cell membrane differentially. Changes in FOS mRNA and protein levels were measured using semi-quantitative RT-PCR and SDS-PAGE Western blotting, respectively, Irradiation of the nucleus or the whole cell at a dose rate of 0.075 Gy/h caused no change in the level of FOS mRNA expression, but modestly (1.5-fold) induced FOS protein after 0.5 h, Irradiation of the nucleus at a dose rate of 0.43 Gy/h induced FOS mRNA by 1.5-fold after 0.5 h, but there was no significant effect after whole-cell irradiation. FOS protein was transiently induced 2.5-fold above control levels 0.5 h after a 0.43-Gy/h exposure of the nucleus or the whole cell. Irradiation of the cell membrane at a dose rate of 1.8 Gy/h for up to 2 h caused no change in the levels of expression of FOS mRNA or protein, but a dose rate of 6.8 Gy/h transiently increased the level of FOS mRNA S-fold after 0.5 h, These data demonstrate the complexity of the cellular response to radiation-induced damage at low doses. The lack of quantitative agreement between the transcript and protein levels for FOS suggests a role for posttranscriptional regulation. (C) 2000 by Radiation Research Society.",
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    Al-Assar, O, Robson, T, McKeown, S, Gardin, I, Wilson, GD & Hirst, DG 2000, 'Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation', Radiation Research, vol. 154, no. 5, pp. 503-514.

    Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation. / Al-Assar, O; Robson, T; McKeown, Stephanie; Gardin, I; Wilson, GD; Hirst, DG.

    In: Radiation Research, Vol. 154, No. 5, 11.2000, p. 503-514.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation

    AU - Al-Assar, O

    AU - Robson, T

    AU - McKeown, Stephanie

    AU - Gardin, I

    AU - Wilson, GD

    AU - Hirst, DG

    PY - 2000/11

    Y1 - 2000/11

    N2 - We irradiated different cellular compartments and measured changes in expression of the FOS gene at the mRNA and protein levels. [H-3]Thymidine and tritiated water were used to irradiate the nucleus and the whole cell, respectively. I-125-Concanavalin A binding was used to irradiate the cell membrane differentially. Changes in FOS mRNA and protein levels were measured using semi-quantitative RT-PCR and SDS-PAGE Western blotting, respectively, Irradiation of the nucleus or the whole cell at a dose rate of 0.075 Gy/h caused no change in the level of FOS mRNA expression, but modestly (1.5-fold) induced FOS protein after 0.5 h, Irradiation of the nucleus at a dose rate of 0.43 Gy/h induced FOS mRNA by 1.5-fold after 0.5 h, but there was no significant effect after whole-cell irradiation. FOS protein was transiently induced 2.5-fold above control levels 0.5 h after a 0.43-Gy/h exposure of the nucleus or the whole cell. Irradiation of the cell membrane at a dose rate of 1.8 Gy/h for up to 2 h caused no change in the levels of expression of FOS mRNA or protein, but a dose rate of 6.8 Gy/h transiently increased the level of FOS mRNA S-fold after 0.5 h, These data demonstrate the complexity of the cellular response to radiation-induced damage at low doses. The lack of quantitative agreement between the transcript and protein levels for FOS suggests a role for posttranscriptional regulation. (C) 2000 by Radiation Research Society.

    AB - We irradiated different cellular compartments and measured changes in expression of the FOS gene at the mRNA and protein levels. [H-3]Thymidine and tritiated water were used to irradiate the nucleus and the whole cell, respectively. I-125-Concanavalin A binding was used to irradiate the cell membrane differentially. Changes in FOS mRNA and protein levels were measured using semi-quantitative RT-PCR and SDS-PAGE Western blotting, respectively, Irradiation of the nucleus or the whole cell at a dose rate of 0.075 Gy/h caused no change in the level of FOS mRNA expression, but modestly (1.5-fold) induced FOS protein after 0.5 h, Irradiation of the nucleus at a dose rate of 0.43 Gy/h induced FOS mRNA by 1.5-fold after 0.5 h, but there was no significant effect after whole-cell irradiation. FOS protein was transiently induced 2.5-fold above control levels 0.5 h after a 0.43-Gy/h exposure of the nucleus or the whole cell. Irradiation of the cell membrane at a dose rate of 1.8 Gy/h for up to 2 h caused no change in the levels of expression of FOS mRNA or protein, but a dose rate of 6.8 Gy/h transiently increased the level of FOS mRNA S-fold after 0.5 h, These data demonstrate the complexity of the cellular response to radiation-induced damage at low doses. The lack of quantitative agreement between the transcript and protein levels for FOS suggests a role for posttranscriptional regulation. (C) 2000 by Radiation Research Society.

    M3 - Article

    VL - 154

    SP - 503

    EP - 514

    JO - Radiation Research

    T2 - Radiation Research

    JF - Radiation Research

    SN - 0033-7587

    IS - 5

    ER -

    Al-Assar O, Robson T, McKeown S, Gardin I, Wilson GD, Hirst DG. Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation. Radiation Research. 2000 Nov;154(5):503-514.